Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0005614
UniProt IDQ9Y6D9
Primary gene name(s)MAD1L1
Synonym gene name(s)MAD1, TXBP181
Protein nameMitotic spindle assembly checkpoint protein MAD1
Protein functionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. {ECO:0000269|PubMed:10049595, ECO:0000269|PubMed:12837246, ECO:0000269|PubMed:20133940}.
Subcellular locationNucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y6D9
Gene Ontology
(Biological Process)
Complete annatation
attachment of mitotic spindle microtubules to kinetochore [GO:0051315];
cell division [GO:0051301];
mitotic cell cycle checkpoint [GO:0007093];
mitotic spindle assembly checkpoint [GO:0007094];
negative regulation of T cell proliferation [GO:0042130];
regulation of metaphase plate congression [GO:0090235];
sister chromatid cohesion [GO:0007062];
thymus development [GO:0048538]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
centrosome [GO:0005813];
condensed chromosome kinetochore [GO:0000777];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
kinetochore [GO:0000776];
mitotic spindle [GO:0072686];
nucleus [GO:0005634];
spindle [GO:0005819];
spindle pole [GO:0000922]
Protein-protein interaction113971
Phylogenetic treeQ9Y6D9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.427145272614492.0277773254862e-050.000116651373472244
AZA vs. DISU-0.2639100583650120.2983946017141310.860938892532141
AZA vs. IL7-0.01539402417016270.936607939637760.999311006273513
AZA vs. SAHA-0.533371171067880.03033151059267960.200886287642378
DISU vs. CD31.152099579037670.00174997547907130.00669521075419308
DISU vs. IL70.2383237774521470.3461633841098860.726969669842606
DISU vs. SAHA-0.2668768719283960.3623661638787430.736837586251643
DMSO vs. AZA-0.06038679885767320.7206839135718821
DMSO vs. CD31.357349290168683.28501228658018e-050.000166684776346684
DMSO vs. DISU0.2021443673503570.4089069625162090.870383340329923
DMSO vs. IL70.05215024191355270.7731431367724960.953700815775867
DMSO vs. SAHA-0.4783660150790780.04398695995585480.234332793741494
HIV vs. Mock in Activation0.2167940339616320.7303480907338090.999983755607037
HIV vs. Mock in Latency-0.06118661359049030.7145930779408970.999834320637052
IL7 vs. CD31.416375934732261.74391719028888e-050.000126072248485488
SAHA vs. CD30.8711057946373590.01527621068936560.0366755607915787
SAHA vs. IL7-0.5198362026318430.03390843907864780.141628099382526
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.409353 0.00241278
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.979 0.986 1.045 1.029 1.026
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1GO4 X-ray 2.0Å E/F/G/H=485-584.
4DZO X-ray 1.7Å A/B=597-718.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)