Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0005525
UniProt IDQ99538
Primary gene name(s)LGMN
Synonym gene name(s)PRSC1
Protein nameLegumain
Protein functionHas a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation, By similarity. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. {ECO:0000250, ECO:0000269|PubMed:23776206}.
Subcellular locationLysosome {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99538
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
negative regulation of ERBB signaling pathway [GO:1901185];
negative regulation of multicellular organism growth [GO:0040015];
negative regulation of neuron apoptotic process [GO:0043524];
proteolysis [GO:0006508];
proteolysis involved in cellular protein catabolic process [GO:0051603];
receptor catabolic process [GO:0032801];
renal system process [GO:0003014];
response to acidic pH [GO:0010447];
toll-like receptor signaling pathway [GO:0002224];
vacuolar protein processing [GO:0006624];
vitamin D metabolic process [GO:0042359]
Gene Ontology
(Molecular Function)
Complete annatation
cysteine-type endopeptidase activity [GO:0004197];
peptidase activity [GO:0008233]
Gene Ontology
(Cellular Component)
Complete annatation
apical part of cell [GO:0045177];
extracellular exosome [GO:0070062];
late endosome [GO:0005770];
lysosomal lumen [GO:0043202];
lysosome [GO:0005764]
Protein-protein interaction111624
Phylogenetic treeQ99538
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.451924807751240.04268371487185840.0874242293421632
AZA vs. DISU0.4584238228507380.2434416843312730.827346002615447
AZA vs. IL7-0.1596784198345330.6900168884787870.999311006273513
AZA vs. SAHA1.401083701045630.0003711238288969240.0102836775676328
DISU vs. CD3-1.008577616300350.1175237513642150.211832240591197
DISU vs. IL7-0.6309544500656720.06826423088662670.34649268707606
DISU vs. SAHA0.9495175000552830.003188100856081450.0469217460957067
DMSO vs. AZA-0.09050981961555110.8459219401020531
DMSO vs. CD3-1.566041739640970.03087825519523480.0643581785440249
DMSO vs. DISU-0.5574347998953450.1817874427918630.696088968126849
DMSO vs. IL7-0.05888865351030290.8902258011561590.97614704263312
DMSO vs. SAHA1.493291973179510.0003799736347664950.00904146144873524
HIV vs. Mock in Activation-0.5172273254742060.6827989131664320.999983755607037
HIV vs. Mock in Latency-0.2566238257810760.4303270836142130.999834320637052
IL7 vs. CD3-1.606761361622610.0193713116048790.0510399933668367
SAHA vs. CD3-0.07029167349874220.9081063629897970.936762467674464
SAHA vs. IL71.565525297986756.02632768476052e-060.000203223408322327
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -1.48469 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.084 1.032 1.104 0.84 1.077
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4AW9 X-ray 2.2Å A=26-309.
4AWA X-ray 2.5Å A=26-309.
4AWB X-ray 2.7Å A/B=26-309.
4FGU X-ray 3.9Å A/B=18-433.
4N6N X-ray 1.8Å A=26-303.
4N6O X-ray 1.8Å A=26-303.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope transmembrane glycoprotein gp41 downregulates 23383108

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)
hsa04612 Antigen processing and presentation - Homo sapiens (human)