Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0005456
UniProt IDP33176
Primary gene name(s)KIF5B
Synonym gene name(s)KNS, KNS1
Protein nameKinesin-1 heavy chain
Protein functionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner, By similarity. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes, PubMed:20386726. {ECO:0000250|UniProtKB:Q61768, ECO:0000269|PubMed:20386726}.
Subcellular locationCytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q2PQA9}. Note=Uniformly distributed between soma and neurites in hippocampal neurons. {ECO:0000250|UniProtKB:Q2PQA9}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P33176
Gene Ontology
(Biological Process)
Complete annatation
axon guidance [GO:0007411];
centrosome localization [GO:0051642];
cytoplasm organization [GO:0007028];
cytoskeleton-dependent intracellular transport [GO:0030705];
microtubule-based movement [GO:0007018];
plus-end-directed vesicle transport along microtubule [GO:0072383];
positive regulation of establishment of protein localization to plasma membrane [GO:0090004];
positive regulation of insulin secretion involved in cellular response to glucose stimulus [GO:0035774];
positive regulation of intracellular protein transport [GO:0090316];
positive regulation of potassium ion transport [GO:0043268];
positive regulation of synaptic transmission, GABAergic [GO:0032230];
positive regulation of vesicle fusion [GO:0031340];
positive regulation of voltage-gated sodium channel activity [GO:1905152];
regulation of membrane potential [GO:0042391];
stress granule disassembly [GO:0035617];
vesicle transport along microtubule [GO:0047496]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
ATP-dependent microtubule motor activity, plus-end-directed [GO:0008574];
microtubule binding [GO:0008017];
microtubule motor activity [GO:0003777]
Gene Ontology
(Cellular Component)
Complete annatation
ciliary rootlet [GO:0035253];
cytoplasm [GO:0005737];
endocytic vesicle [GO:0030139];
kinesin complex [GO:0005871];
membrane [GO:0016020];
microtubule [GO:0005874];
microtubule organizing center [GO:0005815];
neuron projection [GO:0043005];
perinuclear region of cytoplasm [GO:0048471];
vesicle [GO:0031982]
Protein-protein interaction110000
Phylogenetic treeP33176
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4322805335246450.1887931472591350.291679985679856
AZA vs. DISU0.1982783807748410.4345447050577450.915763738642415
AZA vs. IL7-0.1117979393421760.5599960423754160.999311006273513
AZA vs. SAHA-0.07280974439698610.7649655996041370.937311405196428
DISU vs. CD3-0.2470064877757970.4949533096988820.621071022259298
DISU vs. IL7-0.318630687211640.2074960948366340.586369947036804
DISU vs. SAHA-0.2708561391454660.3532847802633580.73062511303574
DMSO vs. AZA-0.09971895117796370.5505405548227361
DMSO vs. CD3-0.5441354594758380.09078654669218430.157878255329304
DMSO vs. DISU-0.3000920458321380.2206835189056520.732779515266557
DMSO vs. IL7-0.00470502936540920.9790748116647280.996407613213289
DMSO vs. SAHA0.01915800752613720.9351323369032680.9846317807625
HIV vs. Mock in Activation0.05076885862979410.935035091549150.999983755607037
HIV vs. Mock in Latency-0.01690230929450360.9181297335246270.999834320637052
IL7 vs. CD3-0.5349478124241050.09744292997796660.186059954846452
SAHA vs. CD3-0.5315064116588620.1338040687501540.22001389155099
SAHA vs. IL70.0341324255037850.8883314388466080.956187928924358
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.223865 0.107341
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.02 1.004 1.09 1.163 1.087
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1BG2 X-ray 1.8Å A=1-325.
1MKJ X-ray 2.7Å A=1-349.
2P4N EM 9.0Å K=1-325.
3J8X EM 5.0Å K=1-349.
3J8Y EM 5.0Å K=1-349.
4HNA X-ray 3.1Å K=1-349.
4LNU X-ray 2.1Å K=1-325.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
capsid regulated by 25231297

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
hsa04728 Dopaminergic synapse - Homo sapiens (human)