Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0005377
UniProt IDP53990
Primary gene name(s)IST1
Synonym gene name(s)KIAA0174
Protein nameIST1 homolog
Protein functionESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells, PubMed:19129479, PubMed:19129480. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing, PubMed:26040712. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST, PubMed:23897888. {ECO:0000269|PubMed:19129479, ECO:0000269|PubMed:19129480, ECO:0000269|PubMed:23897888, ECO:0000269|PubMed:26040712}.
Subcellular locationCytoplasmic vesicle {ECO:0000269|PubMed:19129479, ECO:0000269|PubMed:19129480}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:20719964}. Midbody {ECO:0000269|PubMed:20719964}. Nucleus envelope {ECO:0000269|PubMed:26040712}. Note=Localizes to centrosome and midbody of dividing cells, PubMed:19129480, PubMed:19129479, PubMed:20719964. Colocalized with SPG20 to the ends of Flemming bodies during cytokinesis, PubMed:20719964. Localizes to the nuclear envelope during late anaphase, PubMed:26040712. {ECO:0000269|PubMed:19129479, ECO:0000269|PubMed:19129480, ECO:0000269|PubMed:20719964, ECO:0000269|PubMed:26040712}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P53990
Gene Ontology
(Biological Process)
Complete annatation
abscission [GO:0009838];
cell division [GO:0051301];
cytokinesis [GO:0000910];
ESCRT III complex disassembly [GO:1904903];
establishment of protein localization [GO:0045184];
multivesicular body assembly [GO:0036258];
positive regulation of collateral sprouting [GO:0048672];
positive regulation of proteolysis [GO:0045862];
protein localization [GO:0008104];
protein transport [GO:0015031];
viral capsid secondary envelopment [GO:0046745];
viral release from host cell [GO:0019076]
Gene Ontology
(Molecular Function)
Complete annatation
MIT domain binding [GO:0090541];
protein complex binding [GO:0032403];
protein domain specific binding [GO:0019904]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
cytoplasmic, membrane-bounded vesicle [GO:0016023];
cytosol [GO:0005829];
endoplasmic reticulum-Golgi intermediate compartment [GO:0005793];
extracellular exosome [GO:0070062];
Flemming body [GO:0090543];
midbody [GO:0030496];
nucleus [GO:0005634]
Protein-protein interaction115141
Phylogenetic treeP53990
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1862892294416270.569636908894220.678379226521309
AZA vs. DISU0.1317506189811050.6021120451187460.955158276076142
AZA vs. IL7-0.0911843080652650.6347931561643920.999311006273513
AZA vs. SAHA-0.08198873220179110.7365194674678350.926874494071635
DISU vs. CD30.3051944423338040.4014825767796910.534860659511892
DISU vs. IL7-0.2323275360846180.3560599954574220.734785000892627
DISU vs. SAHA-0.2116342126005960.4678436196954920.802731342023029
DMSO vs. AZA0.03140562578942230.8510121505050991
DMSO vs. CD30.2065231898753950.5187406542861860.625336317261854
DMSO vs. DISU-0.1021048015220540.6752374511498870.955057362910325
DMSO vs. IL7-0.1153523508545230.520543619883140.890304673451612
DMSO vs. SAHA-0.1193593037149270.6122934901681350.871830776400222
HIV vs. Mock in Activation0.05491446815267580.9295929462942750.999983755607037
HIV vs. Mock in Latency0.1052271329963650.5228312347348690.999834320637052
IL7 vs. CD30.1015805723490120.7520692199720020.835059989144603
SAHA vs. CD30.08042726894088490.8197478382744710.872367765185812
SAHA vs. IL70.006309302979721910.9793110340038260.992757870412466
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -1.54108 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.019 0.878 0.857 1.042 0.832
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3FRR X-ray 1.8Å A=1-189.
3FRS X-ray 2.6Å A=5-189.
3JC1 EM 4.0Å Aa/Ac/Ae/Ag/Ai/Ak/Am/Ao/Aq/As/Au/Aw/Ay/Ba/Bc/Be/Bg/Bi/Bk/Bm/Bo/Bq/Bs/Bu/Bw/By/Ca/Cc/Ce/Cg=6-187.
4U7E X-ray 1.6Å A=341-364.
4U7I X-ray 1.7Å B=341-364.
4U7Y X-ray 2.5Å B=341-364.
4WZX X-ray 1.3Å E=342-364.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)