Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004946
UniProt IDO60353
Primary gene name(s)FZD6
Synonym gene name(s)unknown
Protein nameFrizzled-6
Protein functionReceptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity, PCP, particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear, By similarity. {ECO:0000250|UniProtKB:Q61089}.
Subcellular locationMembrane {ECO:0000250|UniProtKB:Q61089};
Multi-pass membrane protein {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:Q61089};
Multi-pass membrane protein {ECO:0000255}. Cell surface {ECO:0000250|UniProtKB:Q61089}. Apical cell membrane;
Multi-pass membrane protein {ECO:0000255}. Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:Q61089};
Multi-pass membrane protein {ECO:0000255}. Note=Colocalizes with FZD3 at the apical face of cells, By similarity. {ECO:0000250|UniProtKB:Q61089}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60353
Gene Ontology
(Biological Process)
Complete annatation
cell proliferation in midbrain [GO:0033278];
embryonic nail plate morphogenesis [GO:0035880];
establishment of planar polarity [GO:0001736];
hair follicle development [GO:0001942];
inner ear morphogenesis [GO:0042472];
midbrain morphogenesis [GO:1904693];
negative regulation of canonical Wnt signaling pathway [GO:0090090];
negative regulation of sequence-specific DNA binding transcription factor activity [GO:0043433];
neural tube closure [GO:0001843];
non-canonical Wnt signaling pathway [GO:0035567];
platelet activation [GO:0030168];
Wnt signaling pathway, calcium modulating pathway [GO:0007223];
Wnt signaling pathway, planar cell polarity pathway [GO:0060071]
Gene Ontology
(Molecular Function)
Complete annatation
G-protein coupled receptor activity [GO:0004930];
ubiquitin protein ligase binding [GO:0031625];
Wnt-activated receptor activity [GO:0042813];
Wnt-protein binding [GO:0017147]
Gene Ontology
(Cellular Component)
Complete annatation
apical plasma membrane [GO:0016324];
apicolateral plasma membrane [GO:0016327];
cell surface [GO:0009986];
cytoplasmic vesicle membrane [GO:0030659];
integral component of membrane [GO:0016021];
integral component of plasma membrane [GO:0005887];
plasma membrane [GO:0005886]
Protein-protein interaction113919
Phylogenetic treeO60353
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5004845517235380.1299117375827420.216884105986607
AZA vs. DISU-0.4025696262618960.282943938910920.851448190328572
AZA vs. IL70.3029960144217740.4019476459312830.999311006273513
AZA vs. SAHA0.1664059417635450.6487429094759360.892443395238835
DISU vs. CD3-0.9158194891768780.01264460598637720.035336446169568
DISU vs. IL70.6976841749060170.06497657729461180.337681329897152
DISU vs. SAHA0.5677188210173590.1383048333323870.477633396689368
DMSO vs. AZA-0.06709616132276870.8333111154894591
DMSO vs. CD3-0.5860543263329490.07001673854050030.127453137519652
DMSO vs. DISU0.3316766772133740.318498767339160.822006745975451
DMSO vs. IL70.378216856209390.2367993808723380.742560382339133
DMSO vs. SAHA0.2265318329822420.4828442447849470.805254911293982
HIV vs. Mock in Activation-0.4600234545711160.4635715629066040.999983755607037
HIV vs. Mock in Latency-0.4607153658364790.006335619435145930.240344647181779
IL7 vs. CD3-0.1862997188811810.578133474705340.698039981609391
SAHA vs. CD3-0.3617643105289780.3088870168828940.42267568731728
SAHA vs. IL7-0.142851802907130.6957291897480840.858356111701867
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander 0.91 6.43E-09 2.57E-07
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.135934 0.411769
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.18 1.476 1.2 1.252 1.302
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04150 mTOR signaling pathway - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa04550 Signaling pathways regulating pluripotency of stem cells - Homo sapiens (human)
hsa04916 Melanogenesis - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05205 Proteoglycans in cancer - Homo sapiens (human)
hsa05217 Basal cell carcinoma - Homo sapiens (human)
hsa05224 Breast cancer - Homo sapiens (human)