Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004863
UniProt IDQ9UKT5
Primary gene name(s)FBXO4
Synonym gene name(s)FBX4
Protein nameF-box only protein 4
Protein functionSubstrate recognition component of a SCF, SKP1-CUL1-F-box protein E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1. {ECO:0000269|PubMed:10531035, ECO:0000269|PubMed:16275645, ECO:0000269|PubMed:18598945, ECO:0000269|PubMed:20159592, ECO:0000269|PubMed:20181953}.
Subcellular locationCytoplasm {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UKT5
Gene Ontology
(Biological Process)
Complete annatation
aging [GO:0007568];
cellular homeostasis [GO:0019725];
cellular response to ionizing radiation [GO:0071479];
common myeloid progenitor cell proliferation [GO:0035726];
negative regulation of fibroblast proliferation [GO:0048147];
negative regulation of protein localization to nucleus [GO:1900181];
positive regulation of protein polyubiquitination [GO:1902916];
positive regulation of protein ubiquitination [GO:0031398];
posttranscriptional regulation of gene expression [GO:0010608];
protein destabilization [GO:0031648];
protein polyubiquitination [GO:0000209];
protein ubiquitination [GO:0016567];
regulation of DNA damage checkpoint [GO:2000001];
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [GO:0031146];
telomere maintenance [GO:0000723];
ubiquitin-dependent protein catabolic process [GO:0006511]
Gene Ontology
(Molecular Function)
Complete annatation
protein homodimerization activity [GO:0042803];
ubiquitin-protein transferase activity [GO:0004842]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
SCF ubiquitin ligase complex [GO:0019005];
ubiquitin ligase complex [GO:0000151]
Protein-protein interaction117656
Phylogenetic treeQ9UKT5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1932279716921910.7516992526227220.827885358825697
AZA vs. DISU-0.9640800012282260.0002510340105232880.0302726933970243
AZA vs. IL70.148159285103650.4575142933550790.999311006273513
AZA vs. SAHA-0.5425270391527620.05947804042312610.298260805501731
DISU vs. CD3-1.16831848175270.07089882160016030.142478181149289
DISU vs. IL71.102460273218012.77096581664527e-050.0019797303168686
DISU vs. SAHA0.421353394988760.1855787534178130.551686629966569
DMSO vs. AZA-0.07682092012985260.6635306001502121
DMSO vs. CD3-0.281236526452230.6440728451377110.734402546047231
DMSO vs. DISU0.8853037699534050.0004828002549118750.0428771661529318
DMSO vs. IL70.2318667536828410.2151452889894870.724804783238115
DMSO vs. SAHA-0.4727966797356340.08702318901488580.344649899705687
HIV vs. Mock in Activation-0.5262573238506990.6798142641167550.999983755607037
HIV vs. Mock in Latency-0.1474959333082270.3913333853065480.999834320637052
IL7 vs. CD3-0.03529960525530790.9541063423282710.972204948370095
SAHA vs. CD3-0.7624954120366090.2363384871142380.34385219162112
SAHA vs. IL7-0.6962475519844710.02103938032852360.104015314425225
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.910128 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.007 1.037 1.281 1.454 1.015
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3L2O X-ray 2.8Å B=55-387.
3L82 X-ray 2.4Å B=162-387.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)