Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004749
UniProt IDQ96HE7
Primary gene name(s)ERO1A
Synonym gene name(s)ERO1L
Protein nameERO1-like protein alpha
Protein functionOxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species, ROS in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1. {ECO:0000269|PubMed:10671517, ECO:0000269|PubMed:10970843, ECO:0000269|PubMed:11707400, ECO:0000269|PubMed:12403808, ECO:0000269|PubMed:18833192, ECO:0000269|PubMed:18971943, ECO:0000269|PubMed:23027870}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:10671517, ECO:0000269|PubMed:14517240};
Peripheral membrane protein {ECO:0000269|PubMed:10671517, ECO:0000269|PubMed:14517240};
Lumenal side {ECO:0000269|PubMed:10671517, ECO:0000269|PubMed:14517240}. Note=The association with ERP44 is essential for its retention in the endoplasmic reticulum.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q96HE7
Gene Ontology
(Biological Process)
Complete annatation
4-hydroxyproline metabolic process [GO:0019471];
animal organ senescence [GO:0010260];
brown fat cell differentiation [GO:0050873];
cell redox homeostasis [GO:0045454];
cellular protein modification process [GO:0006464];
cellular response to hypoxia [GO:0071456];
chaperone mediated protein folding requiring cofactor [GO:0051085];
endoplasmic reticulum unfolded protein response [GO:0030968];
extracellular matrix organization [GO:0030198];
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [GO:0070059];
protein folding [GO:0006457];
protein folding in endoplasmic reticulum [GO:0034975];
protein maturation by protein folding [GO:0022417];
release of sequestered calcium ion into cytosol [GO:0051209];
response to endoplasmic reticulum stress [GO:0034976];
response to reactive oxygen species [GO:0000302];
response to temperature stimulus [GO:0009266]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
Protein-protein interaction119025
Phylogenetic treeQ96HE7
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3352295408688640.3093964964761380.428447629078313
AZA vs. DISU0.1049591908381850.6786809640762710.964884761087644
AZA vs. IL70.1344306677306030.483504360911850.999311006273513
AZA vs. SAHA-0.1243403706047810.6096642987149710.871924064405074
DISU vs. CD30.4282564215903880.2377402461201290.362648904069503
DISU vs. IL70.02044551032419260.9353172945683790.988186499206029
DISU vs. SAHA-0.2285758846857570.4342317048160840.7814874048501
DMSO vs. AZA-0.08649283429114360.604713397874361
DMSO vs. CD30.2386758402442020.4581899508029740.569618056514838
DMSO vs. DISU-0.1928272397083960.4301477934119530.881718877024027
DMSO vs. IL70.2279002499572810.204186934004530.714677973094564
DMSO vs. SAHA-0.04513100478270980.8479255525057040.959461020163348
HIV vs. Mock in Activation-0.05288988505832550.9324023746585210.999983755607037
HIV vs. Mock in Latency0.2611414917266670.1129012718646490.999834320637052
IL7 vs. CD30.4778234507570950.1390562738349990.244345844774571
SAHA vs. CD30.1857379158021640.6020047055492520.699356970125562
SAHA vs. IL7-0.2625487184024930.2806443804674230.527846729433757
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.312664 0.12694
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.053 0.977 1.061 1.075 0.979
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3AHQ X-ray 2.3Å A=22-468.
3AHR X-ray 3.0Å A=22-468.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa05110 Vibrio cholerae infection - Homo sapiens (human)