Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004729
UniProt IDP42566
Primary gene name(s)EPS15
Synonym gene name(s)AF1P
Protein nameEpidermal growth factor receptor substrate 15
Protein functionInvolved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase, RTK type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits, CCPs. Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1, ITGB1 and transferrin receptor, TFR; internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. {ECO:0000269|PubMed:16903783, ECO:0000269|PubMed:18362181, ECO:0000269|PubMed:19458185, ECO:0000269|PubMed:22648170}.
Subcellular locationCytoplasm. Cell membrane;
Peripheral membrane protein;
Cytoplasmic side. Membrane, clathrin-coated pit. Note=Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes. {ECO:0000269|PubMed:16159959}.;
SUBCELLULAR LOCATION: Isoform 2: Early endosome membrane {ECO:0000269|PubMed:18362181};
Peripheral membrane protein {ECO:0000269|PubMed:18362181};
Cytoplasmic side {ECO:0000269|PubMed:18362181}. Note=Colocalizes with HGS on bilayered clathrin coats on endosomes.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P42566
Gene Ontology
(Biological Process)
Complete annatation
cell proliferation [GO:0008283];
clathrin coat assembly [GO:0048268];
endocytic recycling [GO:0032456];
epidermal growth factor receptor signaling pathway [GO:0007173];
Golgi to endosome transport [GO:0006895];
negative regulation of epidermal growth factor receptor signaling pathway [GO:0042059];
positive regulation of receptor recycling [GO:0001921];
protein transport [GO:0015031];
receptor-mediated endocytosis of virus by host cell [GO:0019065];
regulation of cell proliferation [GO:0042127];
vesicle organization [GO:0016050];
viral entry into host cell [GO:0046718]
Gene Ontology
(Molecular Function)
Complete annatation
calcium ion binding [GO:0005509];
polyubiquitin binding [GO:0031593]
Gene Ontology
(Cellular Component)
Complete annatation
aggresome [GO:0016235];
AP-2 adaptor complex [GO:0030122];
ciliary membrane [GO:0060170];
coated pit [GO:0005905];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
early endosome membrane [GO:0031901];
intracellular membrane-bounded organelle [GO:0043231];
membrane [GO:0016020];
plasma membrane [GO:0005886]
Protein-protein interaction108374
Phylogenetic treeP42566
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.225029008765970.4922410703322730.61008572973507
AZA vs. DISU-0.02051288863024450.9353383275635660.995042856396371
AZA vs. IL70.02448800874483160.8986079757737330.999311006273513
AZA vs. SAHA-0.04856958309621280.8420824525580190.962230849025062
DISU vs. CD30.1920852115823270.5958385271963860.706939784254886
DISU vs. IL70.03616195555295890.8857602550294870.979018684989333
DISU vs. SAHA-0.02722405245486950.9255025953718840.982806795139101
DMSO vs. AZA-0.07012741312134930.6754931770730161
DMSO vs. CD30.1441421479267750.6522133595335690.741107198464971
DMSO vs. DISU-0.0512677705115470.8334901937146820.98176467244648
DMSO vs. IL70.1017460985394190.5714046783002040.90371475916411
DMSO vs. SAHA0.01409763197028390.952281685411780.989099014581432
HIV vs. Mock in Activation-0.05496867585296390.9298568558401950.999983755607037
HIV vs. Mock in Latency-0.01346241666540240.9349658517123220.999834320637052
IL7 vs. CD30.2579266736563930.4220221044652470.558631701115717
SAHA vs. CD30.1513021553878420.6684487037180960.754006135107164
SAHA vs. IL7-0.07721745046900520.7510030348756190.888031043906109
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.228592 0.11819
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.985 0.954 1.018 1.145 1
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1C07 NMR - A=217-311.
1EH2 NMR - A=121-218.
1F8H NMR - A=121-215.
1FF1 NMR - A=121-215.
2IV9 X-ray 1.9Å P=723-730.
2JXC NMR - A=121-215.
4RH5 X-ray 1.6Å B=846-854.
4RH9 X-ray 1.6Å B=846-854.
4RHG X-ray 1.5Å B=846-854.
4S0G X-ray 1.7Å B=846-854.
5AWT X-ray 2.7Å B=640-649.
5AWU X-ray 2.7Å B=645-654.
5JP2 X-ray 2.4Å E/F=615-637.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
capsid regulated by 24173214
Pr55(Gag) regulated by 24173214
Nef requires 25496667
HIV-1 virus replication enhanced by expression of human gene 25496667

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)