Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004728
UniProt IDQ9Y6I3
Primary gene name(s)EPN1
Synonym gene name(s)unknown
Protein nameEpsin-1
Protein functionBinds to membranes enriched in phosphatidylinositol 4,5-bisphosphate, PtdIns(4,5P2. Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations, By similarity. Regulates receptor-mediated endocytosis. {ECO:0000250, ECO:0000269|PubMed:10557078}.
Subcellular locationCytoplasm {ECO:0000250}. Cell membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250}. Nucleus {ECO:0000250}. Membrane, clathrin-coated pit {ECO:0000250}. Note=Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y6I3
Gene Ontology
(Biological Process)
Complete annatation
embryonic organ development [GO:0048568];
endocytosis [GO:0006897];
female pregnancy [GO:0007565];
in utero embryonic development [GO:0001701];
negative regulation of epidermal growth factor receptor signaling pathway [GO:0042059];
Notch signaling pathway [GO:0007219]
Gene Ontology
(Molecular Function)
Complete annatation
lipid binding [GO:0008289]
Gene Ontology
(Cellular Component)
Complete annatation
coated pit [GO:0005905];
cytoplasm [GO:0005737];
nucleus [GO:0005634];
plasma membrane [GO:0005886]
Protein-protein interaction118965
Phylogenetic treeQ9Y6I3
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3214333990047880.3329267270371320.453736470739095
AZA vs. DISU-0.1682421753916260.5094358330848860.937979560488626
AZA vs. IL7-0.1233082663199250.5260030540359340.999311006273513
AZA vs. SAHA0.1092256623207380.6569590222392610.896604825824773
DISU vs. CD30.1420532235887140.7171143173537560.801581468395635
DISU vs. IL70.03496659495458170.8983651107139150.981048051481798
DISU vs. SAHA0.279728797310790.3437210788975950.721766760151331
DMSO vs. AZA-0.1617269366093590.3420474529035971
DMSO vs. CD30.1497133642420320.6504429760365610.739778950749173
DMSO vs. DISU0.004860968713196390.9847500883266390.997458249165375
DMSO vs. IL70.04599522171089290.8007764157443580.959270417912966
DMSO vs. SAHA0.2653257064944010.2658252666514430.614495694481925
HIV vs. Mock in Activation0.4063983542137040.5190798564211460.999983755607037
HIV vs. Mock in Latency-0.1550489059222410.3555172857722790.999834320637052
IL7 vs. CD30.202709058702270.552249049180290.67565340245897
SAHA vs. CD30.4075175422150950.2515423214884330.361195120041038
SAHA vs. IL70.2301344412326550.3503687374300370.600029351058893
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.977006 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.996 0.909 1.012 1.244 1.285
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03316 1,4-Diethylene Dioxide experimental unknown unknown
DB03401 D-Myo-Inositol-1,4,5-Triphosphate experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1INZ NMR - A=1-144.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope transmembrane glycoprotein gp41 binds 18375383
Envelope transmembrane glycoprotein gp41 inhibited by 18375383

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)