Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004721
UniProt IDQ09472
Primary gene name(s)EP300
Synonym gene name(s)P300
Protein nameHistone acetyltransferase p300
Protein functionFunctions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122', H3K122ac, a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27', H3K27ac. Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity, PubMed:10733570, PubMed:11430825, PubMed:11701890, PubMed:12402037, PubMed:12586840, PubMed:12929931, PubMed:14645221, PubMed:15186775, PubMed:15890677, PubMed:16617102, PubMed:16762839, PubMed:18722353, PubMed:18995842, PubMed:23415232, PubMed:23911289, PubMed:23934153, PubMed:8945521. Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity, PubMed:20955178. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair, NER, PubMed:24939902. Acetylates MEF2D. {ECO:0000269|PubMed:10733570, ECO:0000269|PubMed:11430825, ECO:0000269|PubMed:11701890, ECO:0000269|PubMed:12402037, ECO:0000269|PubMed:12586840, ECO:0000269|PubMed:12929931, ECO:0000269|PubMed:14645221, ECO:0000269|PubMed:15186775, ECO:0000269|PubMed:15890677, ECO:0000269|PubMed:16617102, ECO:0000269|PubMed:16762839, ECO:0000269|PubMed:18722353, ECO:0000269|PubMed:18995842, ECO:0000269|PubMed:21030595, ECO:0000269|PubMed:23415232, ECO:0000269|PubMed:23911289, ECO:0000269|PubMed:23934153, ECO:0000269|PubMed:24939902, ECO:0000269|PubMed:8945521, ECO:0000305|PubMed:20955178}.
Subcellular locationCytoplasm. Nucleus. Note=In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q09472
Gene Ontology
(Biological Process)
Complete annatation
animal organ morphogenesis [GO:0009887];
apoptotic process [GO:0006915];
B cell differentiation [GO:0030183];
beta-catenin-TCF complex assembly [GO:1904837];
cellular response to UV [GO:0034644];
circadian rhythm [GO:0007623];
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [GO:0006977];
fat cell differentiation [GO:0045444];
heart development [GO:0007507];
histone H2B acetylation [GO:0043969];
histone H4 acetylation [GO:0043967];
internal peptidyl-lysine acetylation [GO:0018393];
internal protein amino acid acetylation [GO:0006475];
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [GO:0042771];
lung development [GO:0030324];
macrophage derived foam cell differentiation [GO:0010742];
megakaryocyte development [GO:0035855];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
nervous system development [GO:0007399];
Notch signaling pathway [GO:0007219];
N-terminal peptidyl-lysine acetylation [GO:0018076];
platelet formation [GO:0030220];
positive regulation by host of viral transcription [GO:0043923];
positive regulation of gene expression, epigenetic [GO:0045815];
positive regulation of protein binding [GO:0032092];
positive regulation of sequence-specific DNA binding transcription factor activity [GO:0051091];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response [GO:0006990];
positive regulation of type I interferon production [GO:0032481];
protein acetylation [GO:0006473];
protein stabilization [GO:0050821];
regulation of androgen receptor signaling pathway [GO:0060765];
regulation of autophagy [GO:0010506];
regulation of cell cycle [GO:0051726];
regulation of cellular response to heat [GO:1900034];
regulation of signal transduction by p53 class mediator [GO:1901796];
regulation of transcription, DNA-templated [GO:0006355];
regulation of transcription from RNA polymerase II promoter in response to hypoxia [GO:0061418];
regulation of tubulin deacetylation [GO:0090043];
response to estrogen [GO:0043627];
response to hypoxia [GO:0001666];
skeletal muscle tissue development [GO:0007519];
somitogenesis [GO:0001756];
stimulatory C-type lectin receptor signaling pathway [GO:0002223];
transcription-coupled nucleotide-excision repair [GO:0006283];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
acetyltransferase activity [GO:0016407];
activating transcription factor binding [GO:0033613];
androgen receptor binding [GO:0050681];
antigen binding [GO:0003823];
beta-catenin binding [GO:0008013];
chromatin binding [GO:0003682];
chromatin DNA binding [GO:0031490];
core promoter binding [GO:0001047];
damaged DNA binding [GO:0003684];
DNA binding [GO:0003677];
histone acetyltransferase activity [GO:0004402];
lysine N-acetyltransferase activity, acting on acetyl phosphate as donor [GO:0004468];
nuclear hormone receptor binding [GO:0035257];
peptide N-acetyltransferase activity [GO:0034212];
pre-mRNA intronic binding [GO:0097157];
protein C-terminus binding [GO:0008022];
RNA polymerase II activating transcription factor binding [GO:0001102];
RNA polymerase II core promoter proximal region sequence-specific DNA binding [GO:0000978];
RNA polymerase II core promoter sequence-specific DNA binding [GO:0000979];
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding [GO:0001228];
transcription coactivator activity [GO:0003713];
transcription factor binding [GO:0008134];
transferase activity, transferring acyl groups [GO:0016746];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
chromatin [GO:0000785];
cytoplasm [GO:0005737];
histone acetyltransferase complex [GO:0000123];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
protein-DNA complex [GO:0032993];
transcription factor complex [GO:0005667]
Protein-protein interaction108347
Phylogenetic treeQ09472
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1914481308716340.5588351462406860.66895110190927
AZA vs. DISU0.3186004346819760.2077640719605960.797384465070977
AZA vs. IL7-0.06894192429822410.7195030152819750.999311006273513
AZA vs. SAHA-0.4061013612281430.09713813074073210.394042029813185
DISU vs. CD30.4964685739023510.1727626359280530.284926036540424
DISU vs. IL7-0.3965356529592370.1154726022023540.445090636205697
DISU vs. SAHA-0.7225959399344860.01357200504565380.126638573484536
DMSO vs. AZA0.0945893190889920.5716735078197411
DMSO vs. CD30.2734799265275280.3933114166276230.507862903188064
DMSO vs. DISU-0.2261089613308640.3534790528395760.845295578681978
DMSO vs. IL7-0.1560559966255170.3847289320627990.836668876348341
DMSO vs. SAHA-0.5066123423745410.03212195223591190.193180344033291
HIV vs. Mock in Activation0.3849036598931940.5388788420761940.999983755607037
HIV vs. Mock in Latency-0.009845076408989850.952314987264920.999834320637052
IL7 vs. CD30.1291309487637260.6880552847907750.787671246876469
SAHA vs. CD3-0.2390552328408950.5018922336878040.610847679024002
SAHA vs. IL7-0.3407262499286980.1624878241152280.383789009826535
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.167275 0.239543
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.993 0.937 0.961 0.948 0.923
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1L3E NMR - B=323-423.
1P4Q NMR - B=323-423.
2K8F NMR - A=1723-1812.
2MH0 NMR - B=1723-1812.
2MZD NMR - A=1723-1812.
3BIY X-ray 1.7Å A=1287-1666.
3I3J X-ray 2.3Å A/B/C/D/E/F/G/H/I/J/K/L=1040-1161.
3IO2 X-ray 2.5Å A=1723-1836.
3P57 X-ray 2.1Å P=1726-1835.
3T92 X-ray 1.5Å A=1723-1818.
4BHW X-ray 2.8Å A/B=1043-1519# A/B=1581-1666.
4PZR X-ray 2.1Å A=1287-1664.
4PZS X-ray 1.9Å A=1287-1664.
4PZT X-ray 2.8Å A=1287-1664.
5BT3 X-ray 1.0Å A=1048-1161.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04024 cAMP signaling pathway - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)
hsa04068 FoxO signaling pathway - Homo sapiens (human)
hsa04110 Cell cycle - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04330 Notch signaling pathway - Homo sapiens (human)
hsa04350 TGF-beta signaling pathway - Homo sapiens (human)
hsa04520 Adherens junction - Homo sapiens (human)
hsa04630 Jak-STAT signaling pathway - Homo sapiens (human)
hsa04720 Long-term potentiation - Homo sapiens (human)
hsa04916 Melanogenesis - Homo sapiens (human)
hsa04919 Thyroid hormone signaling pathway - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)
hsa05016 Huntington's disease - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)
hsa05161 Hepatitis B - Homo sapiens (human)
hsa05164 Influenza A - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05168 Herpes simplex infection - Homo sapiens (human)
hsa05169 Epstein-Barr virus infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)
hsa05211 Renal cell carcinoma - Homo sapiens (human)
hsa05215 Prostate cancer - Homo sapiens (human)