Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004667
UniProt IDQ9H4M9
Primary gene name(s)EHD1
Synonym gene name(s)PAST, PAST1
Protein nameEH domain-containing protein 1
Protein functionATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes, PubMed:24019528. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes, PubMed:15020713, PubMed:17233914, PubMed:20801876. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth, By similarity. Plays a role in myoblast fusion, By similarity. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing, By similarity. Plays a role in the formation of the ciliary vesicle, CV, an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles, DAVs to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis, PubMed:25686250. {ECO:0000250|UniProtKB:Q641Z6, ECO:0000250|UniProtKB:Q9WVK4, ECO:0000269|PubMed:15020713, ECO:0000269|PubMed:17233914, ECO:0000269|PubMed:20801876, ECO:0000269|PubMed:24019528, ECO:0000269|PubMed:25686250}.
Subcellular locationRecycling endosome membrane {ECO:0000269|PubMed:15020713, ECO:0000269|PubMed:17233914, ECO:0000269|PubMed:19864458, ECO:0000269|PubMed:23596323};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Early endosome membrane {ECO:0000269|PubMed:15020713};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Cell membrane {ECO:0000250|UniProtKB:Q9WVK4};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Cell projection, cilium membrane {ECO:0000269|PubMed:25686250};
Peripheral membrane protein {ECO:0000305};
Cytoplasmic side {ECO:0000305}. Note=Preferentially associates with tubular recycling endosomes, PubMed:15020713, PubMed:17233914, PubMed:19864458, PubMed:23596323. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes, By similarity. Localizes to the ciliary pocket from where the cilium protrudes, PubMed:25686250. Colocalizes with BACE1 in tubulovesicular cytoplasmic membranes. Colocalizes with BACE1 and APP amyloid beta proteins in hippocampal mossy fiber terminals, By similarity. {ECO:0000250|UniProtKB:Q9WVK4, ECO:0000269|PubMed:15020713, ECO:0000269|PubMed:17233914, ECO:0000269|PubMed:19864458, ECO:0000269|PubMed:23596323, ECO:0000269|PubMed:25686250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H4M9
Gene Ontology
(Biological Process)
Complete annatation
blood coagulation [GO:0007596];
cellular response to nerve growth factor stimulus [GO:1990090];
cholesterol homeostasis [GO:0042632];
cilium assembly [GO:0060271];
endocytic recycling [GO:0032456];
endocytosis [GO:0006897];
intracellular protein transport [GO:0006886];
low-density lipoprotein particle clearance [GO:0034383];
neuron projection development [GO:0031175];
positive regulation of cholesterol storage [GO:0010886];
positive regulation of endocytic recycling [GO:2001137];
positive regulation of myoblast fusion [GO:1901741];
protein homooligomerization [GO:0051260];
protein localization to cilium [GO:0061512]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
calcium ion binding [GO:0005509];
GTP binding [GO:0005525];
Rab GTPase binding [GO:0017137]
Gene Ontology
(Cellular Component)
Complete annatation
ciliary pocket membrane [GO:0020018];
early endosome membrane [GO:0031901];
endosome membrane [GO:0010008];
extracellular exosome [GO:0070062];
lipid particle [GO:0005811];
membrane [GO:0016020];
plasma membrane [GO:0005886];
platelet dense tubular network membrane [GO:0031095];
recycling endosome membrane [GO:0055038]
Protein-protein interaction116138
Phylogenetic treeQ9H4M9
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.210931659717160.0002643571787321890.00113386209386783
AZA vs. DISU-0.230764501495470.3619312412958530.895413020071178
AZA vs. IL7-0.2524893911382610.1894106622191470.952979086856458
AZA vs. SAHA-0.4693008860437090.05476410971604140.285447506867084
DISU vs. CD30.969118459689220.00817868368525110.0245295419561232
DISU vs. IL7-0.03172660304558760.8996933471170280.981521662424637
DISU vs. SAHA-0.2362525008461820.4176175652063720.771887631044824
DMSO vs. AZA-0.01251413861441370.9404340660292271
DMSO vs. CD31.188005935686270.0002500915127268130.00101479871498672
DMSO vs. DISU0.2165096335004610.3746687352639690.853837959906432
DMSO vs. IL7-0.2326331850215310.1957143689892710.705329694600349
DMSO vs. SAHA-0.4622693882696760.05025738030956860.253254808864779
HIV vs. Mock in Activation0.3585413699988030.566509905248170.999983755607037
HIV vs. Mock in Latency0.00663934777283560.9679109860821630.999834320637052
IL7 vs. CD30.9636804255352590.002999577815422150.0108815009414821
SAHA vs. CD30.7188797542167490.04292612898834410.0874308321304444
SAHA vs. IL7-0.2193767239082630.3678724754085780.617779531066839
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.379719 0.00322755
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.038 1.122 1.182 1.167 1.146
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2JQ6 NMR - A=401-534.
2KFF NMR - A=435-534.
2KFG NMR - A=435-534.
2KFH NMR - A=435-534.
2KSP NMR - A=435-534.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)