Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004651
UniProt IDQ15075
Primary gene name(s)EEA1
Synonym gene name(s)ZFYVE2
Protein nameEarly endosome antigen 1
Protein functionBinds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Subcellular locationCytoplasm. Early endosome membrane;
Peripheral membrane protein.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15075
Gene Ontology
(Biological Process)
Complete annatation
early endosome to late endosome transport [GO:0045022];
endocytosis [GO:0006897];
synaptic vesicle to endosome fusion [GO:0016189];
vesicle fusion [GO:0006906];
viral RNA genome replication [GO:0039694]
Gene Ontology
(Molecular Function)
Complete annatation
1-phosphatidylinositol binding [GO:0005545];
calmodulin binding [GO:0005516];
GTP-dependent protein binding [GO:0030742];
protein homodimerization activity [GO:0042803];
zinc ion binding [GO:0008270]
Gene Ontology
(Cellular Component)
Complete annatation
axonal spine [GO:0044308];
cytoplasm [GO:0005737];
cytoplasmic vesicle [GO:0031410];
cytosol [GO:0005829];
early endosome [GO:0005769];
early endosome membrane [GO:0031901];
extracellular exosome [GO:0070062];
extrinsic component of plasma membrane [GO:0019897];
membrane [GO:0016020];
recycling endosome [GO:0055037];
serine-pyruvate aminotransferase complex [GO:0005969]
Protein-protein interaction113999
Phylogenetic treeQ15075
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.006045704836602590.9853311697395470.990060759354297
AZA vs. DISU-0.2654493996467810.2957574826196690.859421142782033
AZA vs. IL70.1014873994727490.6709895172601230.999311006273513
AZA vs. SAHA-0.09192648104731940.7068551110635310.91681931791089
DISU vs. CD3-0.2712790460041290.4546144604177410.58473452411905
DISU vs. IL70.3585146364068440.1561061317506650.512366630990915
DISU vs. SAHA0.1730043694463420.5541849842683170.853302196353474
DMSO vs. AZA-0.1673260606272890.3205945400943311
DMSO vs. CD3-0.1709454071529070.5945210729104070.691351598872658
DMSO vs. DISU0.09679753750676670.6924932803606030.957954410639349
DMSO vs. IL70.2757088261019490.1450462719191930.646627282384002
DMSO vs. SAHA0.0664877089146180.7783947200366020.93736560220614
HIV vs. Mock in Activation-0.05560911809900370.9293300365543750.999983755607037
HIV vs. Mock in Latency0.0835792486400370.614565331001270.999834320637052
IL7 vs. CD30.1175152117030690.7153353766848980.807598691691368
SAHA vs. CD3-0.1123773842410090.7519508716475540.821865566239603
SAHA vs. IL7-0.1983917114198790.416293438124960.660617674102078
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.212863 0.161413
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.988 1.006 0.964 0.951 1.039
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB02942 Inositol 1,3-Bisphosphate experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1HYI NMR - A=1347-1411.
1HYJ NMR - A=1347-1411.
1JOC X-ray 2.2Å A/B=1287-1324# A/B=1326-1411.
3MJH X-ray 2.0Å B/D=36-69.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu co-localizes with 24086611
Envelope surface glycoprotein gp160; precursor co-localizes with 21789505
Pr55(Gag) co-localizes with 18305041
Vpr upregulates 23874603
Envelope surface glycoprotein gp120 co-localizes with 23729440

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
hsa04145 Phagosome - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)