Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004649
UniProt IDQ92611
Primary gene name(s)EDEM1
Synonym gene name(s)EDEM, KIAA0212
Protein nameER degradation-enhancing alpha-mannosidase-like protein 1
Protein functionExtracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation, ERAD and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2. {ECO:0000269|PubMed:12610306, ECO:0000269|PubMed:19524542, ECO:0000269|PubMed:19934218, ECO:0000269|PubMed:25092655}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:19934218};
Single-pass type II membrane protein {ECO:0000269|PubMed:19934218}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q92611
Gene Ontology
(Biological Process)
Complete annatation
endoplasmic reticulum mannose trimming [GO:1904380];
endoplasmic reticulum unfolded protein response [GO:0030968];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
glycoprotein ERAD pathway [GO:0097466];
IRE1-mediated unfolded protein response [GO:0036498];
mannose trimming involved in glycoprotein ERAD pathway [GO:1904382];
N-glycan processing [GO:0006491];
positive regulation of retrograde protein transport, ER to cytosol [GO:1904154];
trimming of terminal mannose on C branch [GO:0036510]
Gene Ontology
(Molecular Function)
Complete annatation
alpha-mannosidase activity [GO:0004559];
calcium ion binding [GO:0005509];
mannosyl-oligosaccharide 1,2-alpha-mannosidase activity [GO:0004571];
misfolded protein binding [GO:0051787]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum quality control compartment [GO:0044322];
integral component of endoplasmic reticulum membrane [GO:0030176]
Protein-protein interaction115047
Phylogenetic treeQ92611
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.7427179409717670.02609115002715330.0580008574054949
AZA vs. DISU0.003542961565912130.9888193512924380.998932793566471
AZA vs. IL7-0.2091222875692810.2755022671025390.999311006273513
AZA vs. SAHA-0.1362001750949880.5759365291706360.859561599164171
DISU vs. CD30.7330477715645690.04514391645116380.0995318702040139
DISU vs. IL7-0.2211946763175260.3797203693166850.750512348009016
DISU vs. SAHA-0.1390046267857590.6328829450754220.885131669008137
DMSO vs. AZA-0.1322862133037380.4280455405661521
DMSO vs. CD30.5968638805940070.06662140926603510.122426388215762
DMSO vs. DISU-0.138207079584570.571098663023170.930025594527682
DMSO vs. IL7-0.06935449791440880.6988780850501740.937788846060647
DMSO vs. SAHA-0.01084238812443370.9632457137273210.991849854938261
HIV vs. Mock in Activation-0.09627501006484250.8828952544255370.999983755607037
HIV vs. Mock in Latency-0.01096221766684710.9468321390134340.999834320637052
IL7 vs. CD30.5425628291540520.0958282226550070.183464854576263
SAHA vs. CD30.5807217314476080.1045943350055930.180396997747018
SAHA vs. IL70.06822663338903480.7788755311297250.902650092738485
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.274208 0.0362267
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.075 1.003 0.956 0.94 0.803
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
203279_at 1.4 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)