Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004578
UniProt IDP50570
Primary gene name(s)DNM2
Synonym gene name(s)DYN2
Protein nameDynamin-2
Protein functionMicrotubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones, By similarity. Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis, PubMed:12498685. Regulates maturation of apoptotic cell corpse-containing phagosomes by recruiting PIK3C3 to the phagosome membrane, By similarity. {ECO:0000250|UniProtKB:P39052, ECO:0000250|UniProtKB:P39054, ECO:0000269|PubMed:12498685}.
Subcellular locationCytoplasm. Cytoplasm, cytoskeleton. Cell junction {ECO:0000250|UniProtKB:P39052}. Membrane, clathrin-coated pit {ECO:0000250|UniProtKB:P39052}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Midbody. Cell projection, phagocytic cup {ECO:0000250|UniProtKB:P39054}. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:P39054};
Peripheral membrane protein {ECO:0000250|UniProtKB:P39054}. Note=Colocalizes with CTTN at the basis of filopodia in hippocampus neuron growth zones, By similarity. Microtubule-associated. Also found in the postsynaptic density of neuronal cells. Co-localizes with PIK3C3 and RAB5A to the nascent phagosome, By similarity. {ECO:0000250|UniProtKB:P39052, ECO:0000250|UniProtKB:P39054}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P50570
Gene Ontology
(Biological Process)
Complete annatation
antigen processing and presentation of exogenous peptide antigen via MHC class II [GO:0019886];
aorta development [GO:0035904];
cellular response to carbon monoxide [GO:0071245];
cellular response to dopamine [GO:1903351];
cellular response to nitric oxide [GO:0071732];
cellular response to X-ray [GO:0071481];
cilium assembly [GO:0060271];
coronary vasculature development [GO:0060976];
dynamin family protein polymerization involved in mitochondrial fission [GO:0003374];
endocytosis [GO:0006897];
G2/M transition of mitotic cell cycle [GO:0000086];
Golgi to plasma membrane transport [GO:0006893];
G-protein coupled receptor internalization [GO:0002031];
macropinocytosis [GO:0044351];
membrane fusion [GO:0061025];
mitochondrial fission [GO:0000266];
negative regulation of membrane tubulation [GO:1903526];
negative regulation of transforming growth factor beta receptor signaling pathway [GO:0030512];
neuron projection morphogenesis [GO:0048812];
phagocytosis [GO:0006909];
positive regulation of apoptotic process [GO:0043065];
positive regulation of lamellipodium assembly [GO:0010592];
positive regulation of nitric oxide biosynthetic process [GO:0045429];
positive regulation of phagocytosis [GO:0050766];
positive regulation of sodium:potassium-exchanging ATPase activity [GO:1903408];
positive regulation of substrate adhesion-dependent cell spreading [GO:1900026];
positive regulation of transcription, DNA-templated [GO:0045893];
receptor internalization [GO:0031623];
receptor-mediated endocytosis [GO:0006898];
regulation of axon extension [GO:0030516];
regulation of Golgi organization [GO:1903358];
regulation of nitric-oxide synthase activity [GO:0050999];
regulation of Rac protein signal transduction [GO:0035020];
regulation of transcription, DNA-templated [GO:0006355];
response to cocaine [GO:0042220];
response to light stimulus [GO:0009416];
signal transduction [GO:0007165];
spermatogenesis [GO:0007283];
synaptic vesicle transport [GO:0048489];
transferrin transport [GO:0033572];
ventricular septum development [GO:0003281]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
GTPase activity [GO:0003924];
GTP binding [GO:0005525];
microtubule binding [GO:0008017];
SH3 domain binding [GO:0017124]
Gene Ontology
(Cellular Component)
Complete annatation
centrosome [GO:0005813];
clathrin-coated endocytic vesicle [GO:0045334];
coated pit [GO:0005905];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endocytic vesicle membrane [GO:0030666];
endosome [GO:0005768];
extracellular exosome [GO:0070062];
focal adhesion [GO:0005925];
Golgi apparatus [GO:0005794];
Golgi membrane [GO:0000139];
growth cone [GO:0030426];
lamellipodium [GO:0030027];
microtubule [GO:0005874];
midbody [GO:0030496];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
phagocytic cup [GO:0001891];
photoreceptor inner segment [GO:0001917];
plasma membrane [GO:0005886];
postsynaptic density [GO:0014069];
postsynaptic membrane [GO:0045211];
protein complex [GO:0043234];
ruffle membrane [GO:0032587];
trans-Golgi network [GO:0005802]
Protein-protein interaction108122
Phylogenetic treeP50570
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      Yes - >7 SD (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6068102254875530.06457179707586040.122990790538022
AZA vs. DISU0.1004256384142620.6908483299004050.968313969505284
AZA vs. IL7-0.06631566224024260.729482810802350.999311006273513
AZA vs. SAHA-0.1386653851738940.5691407937839880.856334320630286
DISU vs. CD30.6949521173377020.05591340328670390.118077702597895
DISU vs. IL7-0.1761293134639140.4836546724873560.817020863709871
DISU vs. SAHA-0.2369789649436320.4161306533124770.771430478236783
DMSO vs. AZA0.07832691066180170.6390145306804341
DMSO vs. CD30.6721027674039870.03611155678681080.0730915763795921
DMSO vs. DISU-0.02440642679421720.9201461648538930.988899823982894
DMSO vs. IL7-0.1370496843291590.4447106775868330.866711501567098
DMSO vs. SAHA-0.2223308397144410.3453160322345940.696687807470892
HIV vs. Mock in Activation0.2244156288987980.7182438225677820.999983755607037
HIV vs. Mock in Latency-0.02976333326916290.8564484377475870.999834320637052
IL7 vs. CD30.5467796367912090.08916473528483770.173217118505063
SAHA vs. CD30.4444796710344370.2095795217681770.312926126329776
SAHA vs. IL7-0.07534341515940490.756843512864680.890814329110813
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.05635 0.787326
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1 0.922 0.973 1.108 0.945
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2YS1 NMR - A=520-625.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Gag-Pol complexes with 23125841
Envelope transmembrane glycoprotein gp41 interacts with 21338326
Pr55(Gag) co-localizes with 18602423
HIV-1 virus replication enhanced by expression of human gene 18854154
HIV-1 virus replication inhibited by expression of human gene 22082156
Nef complexes with 23125841
Envelope surface glycoprotein gp120 complexes with 23125841
capsid regulated by 23678185
Nef binds 17412836
Nef regulated by 18715908
Pr55(Gag) complexes with 23125841
Pr55(Gag) regulated by 23170932
Nef requires 17412836
Vpu inhibited by 21813615

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)
hsa04666 Fc gamma R-mediated phagocytosis - Homo sapiens (human)
hsa04721 Synaptic vesicle cycle - Homo sapiens (human)
hsa04961 Endocrine and other factor-regulated calcium reabsorption - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)