Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004574
UniProt IDQ13217
Primary gene name(s)DNAJC3
Synonym gene name(s)P58IPK, PRKRI
Protein nameDnaJ homolog subfamily C member 3
Protein functionInvolved in the unfolded protein response, UPR during endoplasmic reticulum, ER stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions, By similarity. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. {ECO:0000250|UniProtKB:Q27968, ECO:0000250|UniProtKB:Q91YW3, ECO:0000269|PubMed:12601012, ECO:0000269|PubMed:8576172, ECO:0000269|PubMed:9447982, ECO:0000269|PubMed:9920933}.
Subcellular locationEndoplasmic reticulum {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13217
Gene Ontology
(Biological Process)
Complete annatation
cellular response to cold [GO:0070417];
defense response to virus [GO:0051607];
IRE1-mediated unfolded protein response [GO:0036498];
negative regulation of apoptotic process [GO:0043066];
negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation [GO:1903912];
positive regulation of translation initiation in response to endoplasmic reticulum stress [GO:0036494];
proteolysis involved in cellular protein catabolic process [GO:0051603]
Gene Ontology
(Molecular Function)
Complete annatation
protein kinase binding [GO:0019901];
protein kinase inhibitor activity [GO:0004860]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum lumen [GO:0005788];
endoplasmic reticulum Sec complex [GO:0031205];
extracellular exosome [GO:0070062];
extracellular vesicle [GO:1903561];
membrane [GO:0016020];
smooth endoplasmic reticulum [GO:0005790]
Protein-protein interaction111597
Phylogenetic treeQ13217
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.05831260277854160.858949947432310.906462232719491
AZA vs. DISU0.08762587080100270.7562000859710860.976021571236163
AZA vs. IL70.1204049059087630.5788705845749690.999311006273513
AZA vs. SAHA-0.2439671917573550.3204028402030070.691397250642243
DISU vs. CD30.134280670485220.7127201634604470.798657727029644
DISU vs. IL70.02463799514206730.9339540525135570.988186499206029
DISU vs. SAHA-0.3316394805019650.2595245875815780.637018739322796
DMSO vs. AZA-0.2260762339490130.1830944709182681
DMSO vs. CD3-0.1774811450414640.5796374308960730.678495563049676
DMSO vs. DISU-0.3152909680013910.2162496850511610.728091984191312
DMSO vs. IL70.3536285634500980.05158140915843210.446403870667897
DMSO vs. SAHA-0.02643828229773690.9112354362987370.979056766269214
HIV vs. Mock in Activation0.09398926031550480.8827491890482460.999983755607037
HIV vs. Mock in Latency0.04070403161874180.8069424805481930.999834320637052
IL7 vs. CD30.1884632435140350.5581416324420870.680277813562007
SAHA vs. CD3-0.2113123319763110.550461117849710.654318429247132
SAHA vs. IL7-0.3690208703958750.1320298920054870.339569799025885
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.193422 0.204415
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.033 1.019 1.285 1.439 1.001
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2Y4T X-ray 3.0Å A/B/C=35-461.
2Y4U X-ray 3.2Å A=35-461.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa05164 Influenza A - Homo sapiens (human)
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