Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004557
UniProt IDQ12959
Primary gene name(s)DLG1
Synonym gene name(s)unknown
Protein nameDisks large homolog 1
Protein functionEssential multidomain scaffolding protein required for normal development, By similarity. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. {ECO:0000250, ECO:0000269|PubMed:10656683, ECO:0000269|PubMed:12445884, ECO:0000269|PubMed:14699157, ECO:0000269|PubMed:15263016, ECO:0000269|PubMed:19213956, ECO:0000269|PubMed:20605917}.
Subcellular locationMembrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250}. Basolateral cell membrane {ECO:0000250}. Endoplasmic reticulum membrane {ECO:0000250}. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density {ECO:0000250}. Cell junction, synapse. Cell membrane, sarcolemma. Note=Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities, By similarity. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q12959
Gene Ontology
(Biological Process)
Complete annatation
actin filament organization [GO:0007015];
activation of protein kinase activity [GO:0032147];
amyloid precursor protein metabolic process [GO:0042982];
astral microtubule organization [GO:0030953];
bicellular tight junction assembly [GO:0070830];
branching involved in ureteric bud morphogenesis [GO:0001658];
cellular protein complex localization [GO:0034629];
chemical synaptic transmission [GO:0007268];
cortical actin cytoskeleton organization [GO:0030866];
cortical microtubule organization [GO:0043622];
embryonic skeletal system morphogenesis [GO:0048704];
endothelial cell proliferation [GO:0001935];
establishment of centrosome localization [GO:0051660];
establishment or maintenance of cell polarity [GO:0007163];
establishment or maintenance of epithelial cell apical/basal polarity [GO:0045197];
hard palate development [GO:0060022];
immunological synapse formation [GO:0001771];
lens development in camera-type eye [GO:0002088];
membrane raft organization [GO:0031579];
mitotic cell cycle checkpoint [GO:0007093];
negative regulation of epithelial cell proliferation [GO:0050680];
negative regulation of ERK1 and ERK2 cascade [GO:0070373];
negative regulation of mitotic cell cycle [GO:0045930];
negative regulation of p38MAPK cascade [GO:1903753];
negative regulation of protein kinase B signaling [GO:0051898];
negative regulation of T cell proliferation [GO:0042130];
negative regulation of transcription from RNA polymerase II promoter [GO:0000122];
nervous system development [GO:0007399];
peristalsis [GO:0030432];
positive regulation of actin filament polymerization [GO:0030838];
positive regulation of cell proliferation [GO:0008284];
positive regulation of establishment of protein localization to plasma membrane [GO:0090004];
positive regulation of potassium ion transport [GO:0043268];
protein localization to plasma membrane [GO:0072659];
receptor clustering [GO:0043113];
receptor localization to synapse [GO:0097120];
regulation of cell shape [GO:0008360];
regulation of membrane potential [GO:0042391];
regulation of myelination [GO:0031641];
regulation of potassium ion export across plasma membrane [GO:1903764];
regulation of potassium ion import [GO:1903286];
regulation of sodium ion transmembrane transport [GO:1902305];
regulation of ventricular cardiac muscle cell action potential [GO:0098911];
regulation of voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization [GO:1903760];
reproductive structure development [GO:0048608];
single organismal cell-cell adhesion [GO:0016337];
smooth muscle tissue development [GO:0048745];
T cell activation [GO:0042110];
T cell cytokine production [GO:0002369];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
cytoskeletal protein binding [GO:0008092];
guanylate kinase activity [GO:0004385];
ion channel binding [GO:0044325];
ionotropic glutamate receptor binding [GO:0035255];
L27 domain binding [GO:0097016];
ligand-gated ion channel activity [GO:0015276];
mitogen-activated protein kinase kinase binding [GO:0031434];
phosphatase binding [GO:0019902];
phosphoprotein phosphatase activity [GO:0004721];
potassium channel regulator activity [GO:0015459];
protein C-terminus binding [GO:0008022];
protein kinase binding [GO:0019901]
Gene Ontology
(Cellular Component)
Complete annatation
basal lamina [GO:0005605];
basolateral plasma membrane [GO:0016323];
bicellular tight junction [GO:0005923];
cell-cell junction [GO:0005911];
cell junction [GO:0030054];
cell projection membrane [GO:0031253];
cytoplasm [GO:0005737];
cytoplasmic side of plasma membrane [GO:0009898];
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
extracellular exosome [GO:0070062];
Golgi apparatus [GO:0005794];
immunological synapse [GO:0001772];
intercalated disc [GO:0014704];
lateral loop [GO:0043219];
lateral plasma membrane [GO:0016328];
membrane raft [GO:0045121];
microtubule [GO:0005874];
MPP7-DLG1-LIN7 complex [GO:0097025];
myelin sheath abaxonal region [GO:0035748];
neuromuscular junction [GO:0031594];
node of Ranvier [GO:0033268];
nucleus [GO:0005634];
perinuclear region of cytoplasm [GO:0048471];
plasma membrane [GO:0005886];
postsynaptic density [GO:0014069];
postsynaptic membrane [GO:0045211];
sarcolemma [GO:0042383]
Protein-protein interaction108083
Phylogenetic treeQ12959
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.1778994872050330.5872410225504830.693643941369976
AZA vs. DISU0.05760174120543230.8198739208779610.984374783402915
AZA vs. IL70.08325624426680820.6653872360315550.999311006273513
AZA vs. SAHA0.01892979592325490.9381750558417050.986731349623563
DISU vs. CD30.2226626398731010.5391202894224670.659279955520669
DISU vs. IL70.01672602219692190.9470535995140550.991053757168342
DISU vs. SAHA-0.037849821556680.896608633963470.974386264959868
DMSO vs. AZA-0.0542057320568850.7468692463804761
DMSO vs. CD30.1128138442792430.7243994197728070.799200092191677
DMSO vs. DISU-0.1135846256193140.6416046474977710.9472626202031
DMSO vs. IL70.1446602516079970.4218427907971640.854003087820276
DMSO vs. SAHA0.06577292640569580.7803413874562770.937980450633696
HIV vs. Mock in Activation0.09531984234627870.878504313216520.999983755607037
HIV vs. Mock in Latency0.01571934874964180.9241966270541950.999834320637052
IL7 vs. CD30.2693895828610430.4021493433150850.539240018840697
SAHA vs. CD30.1713537928274240.6277531073036350.720780139205012
SAHA vs. IL7-0.06832925649156920.7790648312810950.902740347476808
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.149423 0.336094
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.966 0.954 0.868 0.831 1.215
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1PDR X-ray 2.8Å A=457-555.
2M3M NMR - A=318-406.
2OQS NMR - A=318-406.
2X7Z X-ray 2.0Å A=311-407.
3LRA X-ray 2.9Å A=2-65.
3RL7 X-ray 2.3Å A/B/C/D/E/F=220-317.
3RL8 X-ray 2.2Å A/B/C/D/E=315-410.
3W9Y X-ray 2.2Å A=712-904.
4AMH X-ray 2.3Å A/B=315-405.
4G69 X-ray 2.0Å A=310-407.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp160; precursor inhibited by 18946087
21762802
nucleocapsid interacts with 18946087
21762802
22004035
Pr55(Gag) interacts with 18946087
21762802
2200403518946087
22004035

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa04530 Tight junction - Homo sapiens (human)
hsa04660 T cell receptor signaling pathway - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
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