Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004523
UniProt IDQ9BUN8
Primary gene name(s)DERL1
Synonym gene name(s)DER1
Protein nameDerlin-1
Protein functionFunctional component of endoplasmic reticulum-associated degradation, ERAD for misfolded lumenal proteins. May act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and the degradation substrate. In case of infection by cytomegaloviruses, it plays a central role in the export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Also participates in the degradation process of misfolded cytomegalovirus US2 protein. {ECO:0000269|PubMed:15215855, ECO:0000269|PubMed:15215856}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:15215855, ECO:0000269|PubMed:15215856, ECO:0000269|PubMed:16186509, ECO:0000269|PubMed:16449189};
Multi-pass membrane protein {ECO:0000269|PubMed:15215855, ECO:0000269|PubMed:15215856, ECO:0000269|PubMed:16186509, ECO:0000269|PubMed:16449189}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9BUN8
Gene Ontology
(Biological Process)
Complete annatation
endoplasmic reticulum unfolded protein response [GO:0030968];
ERAD pathway [GO:0036503];
ER-associated misfolded protein catabolic process [GO:0071712];
ER-associated ubiquitin-dependent protein catabolic process [GO:0030433];
establishment of protein localization [GO:0045184];
intracellular transport of viral protein in host cell [GO:0019060];
positive regulation of protein binding [GO:0032092];
positive regulation of protein ubiquitination [GO:0031398];
protein destabilization [GO:0031648];
protein homooligomerization [GO:0051260];
response to unfolded protein [GO:0006986];
retrograde protein transport, ER to cytosol [GO:0030970]
Gene Ontology
(Molecular Function)
Complete annatation
ATPase binding [GO:0051117];
MHC class I protein binding [GO:0042288];
protease binding [GO:0002020];
receptor activity [GO:0004872];
ubiquitin protein ligase binding [GO:0031625];
ubiquitin-specific protease binding [GO:1990381]
Gene Ontology
(Cellular Component)
Complete annatation
Derlin-1 retrotranslocation complex [GO:0036513];
Derlin-1-VIMP complex [GO:0036502];
early endosome [GO:0005769];
endoplasmic reticulum [GO:0005783];
host cell [GO:0043657];
integral component of endoplasmic reticulum membrane [GO:0030176];
integral component of membrane [GO:0016021];
late endosome [GO:0005770];
membrane [GO:0016020]
Protein-protein interaction122559
Phylogenetic treeQ9BUN8
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.2914065113845290.3733983453181320.495322994247041
AZA vs. DISU0.2491631049104390.3249590446947760.874426888925675
AZA vs. IL70.06445128856036870.7375123848719780.999311006273513
AZA vs. SAHA-0.2824480078462460.2479050661750320.618832313522503
DISU vs. CD3-0.05472971191891360.8800325404362460.919688262232857
DISU vs. IL7-0.1938868227983510.4414029511931750.790790328954956
DISU vs. SAHA-0.530065191719670.06945444090497030.33240643978368
DMSO vs. AZA-0.01132274649354230.946160949222771
DMSO vs. CD3-0.3128240171271250.3281385975047160.441691205451086
DMSO vs. DISU-0.2620062420352750.2829623103529560.798111145486858
DMSO vs. IL70.08290024079486720.6448255970333710.924086867864902
DMSO vs. SAHA-0.2779209445992970.2389935667308170.586815256467928
HIV vs. Mock in Activation0.05894817657816910.9244894947795350.999983755607037
HIV vs. Mock in Latency0.09203747120165740.5771004695980260.999834320637052
IL7 vs. CD3-0.2196559330008590.494120871271270.624922909433056
SAHA vs. CD3-0.5980829434477050.09195674346350590.162746696887262
SAHA vs. IL7-0.3501597994498840.1510370802351910.368143776757594
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.134977 0.37046
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.08 1.105 1.003 1.028 1.357
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)
hsa05014 Amyotrophic lateral sclerosis (ALS) - Homo sapiens (human)