Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004464
UniProt IDP61803
Primary gene name(s)DAD1
Synonym gene name(s)unknown
Protein nameDolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Protein functionEssential subunit of the N-oligosaccharyl transferase, OST complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum, ER. Loss of the DAD1 protein triggers apoptosis, By similarity. {ECO:0000250}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000250};
Multi-pass membrane protein {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P61803
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
blastocyst development [GO:0001824];
negative regulation of apoptotic process [GO:0043066];
protein glycosylation [GO:0006486];
protein N-linked glycosylation [GO:0006487];
protein N-linked glycosylation via asparagine [GO:0018279];
response to drug [GO:0042493];
response to nutrient [GO:0007584]
Gene Ontology
(Molecular Function)
Complete annatation
dolichyl-diphosphooligosaccharide-protein glycotransferase activity [GO:0004579]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum membrane [GO:0005789];
extracellular exosome [GO:0070062];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
oligosaccharyltransferase complex [GO:0008250]
Protein-protein interaction107973
Phylogenetic treeP61803
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.001796335884583540.9956880211846160.996708733965682
AZA vs. DISU-0.4110281207659880.1051666255368610.653313462609335
AZA vs. IL7-0.07620673161407590.6916196006617780.999311006273513
AZA vs. SAHA0.1162985057141120.6346276266081990.884792530844617
DISU vs. CD3-0.4221749165542520.2584401388200890.386097785190686
DISU vs. IL70.3256052191548570.1970248441314260.571622310485228
DISU vs. SAHA0.5291218460612240.0699551144575510.333738297801992
DMSO vs. AZA-0.05058533568821810.7625468879901011
DMSO vs. CD3-0.06254071935538640.8495927606405170.896610503611165
DMSO vs. DISU0.357927171552730.1430236293900940.635827251916125
DMSO vs. IL7-0.01809080056674870.9198059823038440.98354773913261
DMSO vs. SAHA0.1613572707701750.4945907350460670.811247438822671
HIV vs. Mock in Activation-0.2959655222761010.6901630330887370.999983755607037
HIV vs. Mock in Latency-0.2239057782112020.1755456983077270.999834320637052
IL7 vs. CD3-0.06681542639950410.8399680223822580.896659915111032
SAHA vs. CD30.09367475793855380.7997586278280980.8577796824806
SAHA vs. IL70.1896231966377150.4376580698750160.678023584043544
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0354588 0.859653
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.046 1.069 1.151 1.181 1.009
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 19460752

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00510 N-Glycan biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)