Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004437
UniProt IDQ93034
Primary gene name(s)CUL5
Synonym gene name(s)VACM1
Protein nameCullin-5
Protein functionCore component of multiple SCF-like ECS, Elongin-Cullin 2/5-SOCS-box protein E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1 seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q93034
Gene Ontology
(Biological Process)
Complete annatation
cell cycle arrest [GO:0007050];
cell proliferation [GO:0008283];
ERBB2 signaling pathway [GO:0038128];
G1/S transition of mitotic cell cycle [GO:0000082];
intrinsic apoptotic signaling pathway [GO:0097193];
negative regulation of cell proliferation [GO:0008285];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
calcium channel activity [GO:0005262];
receptor activity [GO:0004872];
ubiquitin protein ligase binding [GO:0031625];
ubiquitin-protein transferase activity [GO:0004842]
Gene Ontology
(Cellular Component)
Complete annatation
Cul5-RING ubiquitin ligase complex [GO:0031466];
cytosol [GO:0005829]
Protein-protein interaction113743
Phylogenetic treeQ93034
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7598880226481360.02096954716442820.0484520948738713
AZA vs. DISU0.100802417500830.6911700276192790.968313969505284
AZA vs. IL70.1559501512561930.4399965694093780.999311006273513
AZA vs. SAHA-0.4755194113843830.05353576964747410.281754719624777
DISU vs. CD3-0.6719156115690580.06511056639133190.133443464008557
DISU vs. IL70.04671210034514570.8531701049964550.970688298887122
DISU vs. SAHA-0.5764330867164140.04908788709233480.276458724352981
DMSO vs. AZA-0.1662978986283320.3243765753560141
DMSO vs. CD3-0.9365953570215610.003670914982959350.0105579103127687
DMSO vs. DISU-0.2686886978580410.2728239247939010.79139779916016
DMSO vs. IL70.3292793326809680.0686614816463340.50100282469433
DMSO vs. SAHA-0.3176128086906020.181847081472980.514669927676862
HIV vs. Mock in Activation-0.1809375125768860.7719694519699140.999983755607037
HIV vs. Mock in Latency-0.03592164470506720.8284632965408910.999834320637052
IL7 vs. CD3-0.5943316130139250.06512180427397680.135720632157863
SAHA vs. CD3-1.26160769690530.000455830603512730.00180820405236558
SAHA vs. IL7-0.6361544315624580.009641765131706670.0608626329963762
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.111378 0.496682
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.988 0.993 1.08 1.181 1.117
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3DPL X-ray 2.6Å C=401-780.
3DQV X-ray 3.0Å C/D=401-780.
4JGH X-ray 3.0Å D=10-386.
4N9F X-ray 3.3Å 3/9/C/I/O/U/V/f/l/r/w/x=12-321.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)