Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004436
UniProt IDQ13620
Primary gene name(s)CUL4B
Synonym gene name(s)KIAA0695
Protein nameCullin-4B
Protein functionCore component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin, mTOR pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. {ECO:0000269|PubMed:14578910, ECO:0000269|PubMed:16322693, ECO:0000269|PubMed:16678110, ECO:0000269|PubMed:18235224, ECO:0000269|PubMed:18593899, ECO:0000269|PubMed:19801544, ECO:0000269|PubMed:22118460}.
Subcellular locationNucleus {ECO:0000269|PubMed:18593899, ECO:0000269|PubMed:19801544}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q13620
Gene Ontology
(Biological Process)
Complete annatation
cell cycle [GO:0007049];
DNA damage response, detection of DNA damage [GO:0042769];
global genome nucleotide-excision repair [GO:0070911];
histone H2A monoubiquitination [GO:0035518];
neuron projection development [GO:0031175];
nucleotide-excision repair, DNA damage recognition [GO:0000715];
nucleotide-excision repair, DNA duplex unwinding [GO:0000717];
nucleotide-excision repair, DNA incision [GO:0033683];
nucleotide-excision repair, DNA incision, 3'-to lesion [GO:0006295];
nucleotide-excision repair, DNA incision, 5'-to lesion [GO:0006296];
nucleotide-excision repair, preincision complex assembly [GO:0006294];
nucleotide-excision repair, preincision complex stabilization [GO:0006293];
positive regulation of G1/S transition of mitotic cell cycle [GO:1900087];
positive regulation of protein catabolic process [GO:0045732];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
transcription-coupled nucleotide-excision repair [GO:0006283];
UV-damage excision repair [GO:0070914]
Gene Ontology
(Molecular Function)
Complete annatation
ubiquitin protein ligase binding [GO:0031625]
Gene Ontology
(Cellular Component)
Complete annatation
Cul4B-RING E3 ubiquitin ligase complex [GO:0031465];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
nucleoplasm [GO:0005654]
Protein-protein interaction114028
Phylogenetic treeQ13620
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.330451838251156.86761595541929e-050.000345835976211425
AZA vs. DISU0.05991368113210410.8125887023978010.983626273983733
AZA vs. IL7-0.2232065878072850.2458415296669630.995802196416289
AZA vs. SAHA-0.1129210773967320.6434021658034490.889611842976484
DISU vs. CD31.378256077041870.0001943082840012430.00101419773997048
DISU vs. IL7-0.2921622807411060.2461241055331360.632297357006139
DISU vs. SAHA-0.1726300655451270.5535750653662550.853060307784748
DMSO vs. AZA-0.1419790506718820.3967877195747621
DMSO vs. CD31.176641141188550.0003044154790602380.00120304805667432
DMSO vs. DISU-0.2039830231505190.4030738431079940.866398918219579
DMSO vs. IL7-0.07395194433644340.6811001477153040.934977448789731
DMSO vs. SAHA0.02168541564884380.9266681241709040.982780458369212
HIV vs. Mock in Activation-0.2224659634954850.7221430288389450.999983755607037
HIV vs. Mock in Latency0.04414215067591750.7890926140174310.999834320637052
IL7 vs. CD31.116111881290680.0006115271968045730.00278728437781534
SAHA vs. CD31.191680852386950.0009515273069355960.00343297019763453
SAHA vs. IL70.1057045539268460.6645618100804740.838293282439587
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.419976 0.00154878
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.011 1.007 1.126 1.104 1.121
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2DO7 NMR - A=826-913.
4A0C X-ray 3.8Å C/E=192-913.
4A0L X-ray 7.4Å E/H=192-913.
4A64 X-ray 2.5Å A/B/C/D=206-557.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpr interacts with 24719410
Rev interacts with 19339032
Vpr complexes with 24719410

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa03420 Nucleotide excision repair - Homo sapiens (human)
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)