Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004404
UniProt IDQ53ET0
Primary gene name(s)CRTC2
Synonym gene name(s)TORC2
Protein nameCREB-regulated transcription coactivator 2
Protein functionTranscriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element, CRE sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1, HTLV-1 long terminal repeats, LTR. {ECO:0000269|PubMed:14506290, ECO:0000269|PubMed:14536081, ECO:0000269|PubMed:15454081, ECO:0000269|PubMed:16809310, ECO:0000269|PubMed:16817901, ECO:0000269|PubMed:16980408, ECO:0000269|PubMed:17210223}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:15589160}. Nucleus {ECO:0000269|PubMed:15589160}. Note=Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein, PubMed:15454081. In response to cAMP levels and glucagon, relocated to the nucleus, PubMed:15454081. {ECO:0000269|PubMed:15454081}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q53ET0
Gene Ontology
(Biological Process)
Complete annatation
gluconeogenesis [GO:0006094];
glucose homeostasis [GO:0042593];
histone H3-K9 acetylation [GO:0043970];
positive regulation of CREB transcription factor activity [GO:0032793];
positive regulation of transcription from RNA polymerase II promoter [GO:0045944];
protein homotetramerization [GO:0051289];
toxin transport [GO:1901998];
transcription, DNA-templated [GO:0006351];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
cAMP response element binding protein binding [GO:0008140];
chromatin binding [GO:0003682]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
extracellular exosome [GO:0070062];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction128308
Phylogenetic treeQ53ET0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.4666354142858970.1548242454525780.249413320592267
AZA vs. DISU0.4433222098643380.08074157908738750.599260740109936
AZA vs. IL7-0.190579592780310.3253747014586680.999311006273513
AZA vs. SAHA0.1615417923293050.5089813574861410.826533098527826
DISU vs. CD30.8968497289024650.01423172346094970.0389486200890261
DISU vs. IL7-0.6435352402502490.01098201387953720.124476585890243
DISU vs. SAHA-0.2786157661641110.3433418827732230.721487104751149
DMSO vs. AZA0.07808716443173630.641663497113511
DMSO vs. CD30.5326272461010260.0965243986025560.166111900131987
DMSO vs. DISU-0.3671465445839430.1334269157319720.623975061846561
DMSO vs. IL7-0.261168467233980.1472213536389840.649385638582177
DMSO vs. SAHA0.07884319494245040.7386783552833250.924026643277682
HIV vs. Mock in Activation0.237319145601240.7032368503618250.999983755607037
HIV vs. Mock in Latency0.08841715870041620.5925162662126450.999834320637052
IL7 vs. CD30.2808375610107970.3824339405367740.519083906862746
SAHA vs. CD30.6051926494526720.08828944679229610.157551446200107
SAHA vs. IL70.3497953561651130.1533505280727120.371044279320717
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.3481 0.03638

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0758348 0.66759
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.085 0.532 0.812 0.952 0.854
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4HTM X-ray 2.0Å A=18-50.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04152 AMPK signaling pathway - Homo sapiens (human)
hsa04922 Glucagon signaling pathway - Homo sapiens (human)
hsa04931 Insulin resistance - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)