Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004294
UniProt IDO75503
Primary gene name(s)CLN5
Synonym gene name(s)unknown
Protein nameCeroid-lipofuscinosis neuronal protein 5
Protein functionPlays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane, PubMed:22431521. {ECO:0000269|PubMed:22431521}.
Subcellular locationCeroid-lipofuscinosis neuronal protein 5, secreted form: Lysosome {ECO:0000269|PubMed:11971870, ECO:0000269|PubMed:20052765, ECO:0000269|PubMed:22431521, ECO:0000269|PubMed:24038957, ECO:0000269|PubMed:24058541}.;
SUBCELLULAR LOCATION: Ceroid-lipofuscinosis neuronal protein 5: Membrane {ECO:0000269|PubMed:24038957};
Single-pass type II membrane protein {ECO:0000269|PubMed:24038957}. Note=An amphipathic anchor region facilitates its association with the membrane. {ECO:0000269|PubMed:24038957}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O75503
Gene Ontology
(Biological Process)
Complete annatation
brain development [GO:0007420];
glycosylation [GO:0070085];
lysosomal lumen acidification [GO:0007042];
neurogenesis [GO:0022008];
neuron maturation [GO:0042551];
positive regulation of GTP binding [GO:1904426];
protein catabolic process [GO:0030163];
retrograde transport, endosome to Golgi [GO:0042147];
signal peptide processing [GO:0006465]
Gene Ontology
(Molecular Function)
Complete annatation
mannose binding [GO:0005537]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
extracellular exosome [GO:0070062];
Golgi apparatus [GO:0005794];
integral component of membrane [GO:0016021];
lysosomal membrane [GO:0005765];
lysosome [GO:0005764];
perinuclear region of cytoplasm [GO:0048471]
Protein-protein interaction107614
Phylogenetic treeO75503
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.3097276848724070.3480452837083960.468411332802298
AZA vs. DISU-0.2370807144880440.3522263264218180.888688921219146
AZA vs. IL7-0.1313526249398690.4995948716738640.999311006273513
AZA vs. SAHA-0.341149388669160.1662293687046340.519963699176815
DISU vs. CD30.06023832308815450.8694176186374380.911884301651875
DISU vs. IL70.09662631768512710.7031927546161740.923741138299926
DISU vs. SAHA-0.103238930038920.7255503181863290.919234926748118
DMSO vs. AZA-0.1113794121624840.5130909633691681
DMSO vs. CD30.1873436850910470.5617350083204070.66381918180228
DMSO vs. DISU0.1240525845105530.6134815862136570.939338220488525
DMSO vs. IL7-0.01301211107117690.9430523940651120.98914572901033
DMSO vs. SAHA-0.2368685733886920.320277503770550.673395962517573
HIV vs. Mock in Activation-0.3018817680889350.6287186022591010.999983755607037
HIV vs. Mock in Latency0.04398992681814490.7923534921996650.999834320637052
IL7 vs. CD30.1869124435073280.563689655725730.684704180897247
SAHA vs. CD3-0.05635335753896710.8737379217799930.911668661720176
SAHA vs. IL7-0.2138597140120640.3843111048416930.634255163710988
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.433331 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.984 0.996 1.482 1.539 1.089
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
214252_s_at 1.48 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)