Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004256
UniProt IDQ9Y3E7
Primary gene name(s)CHMP3
Synonym gene name(s)CGI149, NEDF, VPS24
Protein nameCharged multivesicular body protein 3
Protein functionProbable core component of the endosomal sorting required for transport complex III, ESCRT-III which is involved in multivesicular bodies, MVBs formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles, ILVs that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses, HIV-1 and other lentiviruses. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5P2 and PtdIns(3,4P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress-mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells. {ECO:0000269|PubMed:14505570, ECO:0000269|PubMed:15707591, ECO:0000269|PubMed:16740483, ECO:0000269|PubMed:17331679, ECO:0000269|PubMed:18076377}.
Subcellular locationCytoplasm, cytosol. Membrane;
Lipid-anchor. Endosome. Late endosome membrane {ECO:0000305}. Note=Localizes to the midbody of dividing cells.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9Y3E7
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
autophagosome maturation [GO:0097352];
autophagy [GO:0006914];
cell cycle [GO:0007049];
cell separation after cytokinesis [GO:0000920];
endosomal transport [GO:0016197];
multivesicular body assembly [GO:0036258];
multivesicular body-lysosome fusion [GO:0061763];
multivesicular body sorting pathway [GO:0071985];
negative regulation of viral release from host cell [GO:1902187];
positive regulation of viral release from host cell [GO:1902188];
protein heterooligomerization [GO:0051291];
protein polymerization [GO:0051258];
protein transport [GO:0015031];
regulation of centrosome duplication [GO:0010824];
regulation of early endosome to late endosome transport [GO:2000641];
regulation of viral process [GO:0050792];
viral budding via host ESCRT complex [GO:0039702];
viral life cycle [GO:0019058]
Gene Ontology
(Molecular Function)
Complete annatation
phosphatidylcholine binding [GO:0031210];
protein homodimerization activity [GO:0042803];
ubiquitin-specific protease binding [GO:1990381]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasmic, membrane-bounded vesicle [GO:0016023];
cytosol [GO:0005829];
ESCRT III complex [GO:0000815];
extracellular exosome [GO:0070062];
late endosome membrane [GO:0031902];
plasma membrane [GO:0005886]
Protein-protein interaction119660;1529307
Phylogenetic treeQ9Y3E7
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.594083895789440.07138709124915490.133409329114354
AZA vs. DISU0.007268448292700390.9770956404173530.997889048290254
AZA vs. IL7-0.1285298273580230.5052038263803850.999311006273513
AZA vs. SAHA-0.755912130470020.002235939382488230.035970704644213
DISU vs. CD30.5886964595646970.1064391057780520.195737840734214
DISU vs. IL7-0.1449176122383080.565507223868760.860337501549187
DISU vs. SAHA-0.7620884931874890.009840624812543110.102281418310233
DMSO vs. AZA-0.0762878364988470.6503369313598381
DMSO vs. CD30.5063778687646920.1157040076455590.191948119720333
DMSO vs. DISU-0.08553460697577970.7261655334722040.964942874095702
DMSO vs. IL7-0.04496683222313210.8032016426493410.960287474259562
DMSO vs. SAHA-0.6863604609858710.004111949705532520.048414995321938
HIV vs. Mock in Activation0.02918103966296780.9626986032285630.999983755607037
HIV vs. Mock in Latency0.08534938008699310.6061835428538970.999834320637052
IL7 vs. CD30.4733307354643640.1427733763437430.249365365194582
SAHA vs. CD3-0.1866689235793140.5978218730725760.695552349882426
SAHA vs. IL7-0.6312494070879930.01046620923494650.0644972453899929
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.443377 0.0784545
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.97 1.084 1.036 1.117 1.233
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2GD5 X-ray 2.8Å A/B/C/D=9-183.
2XZE X-ray 1.7Å Q/R=183-222.
3FRT X-ray 4.0Å A/B=8-222.
3FRV X-ray 3.7Å A=1-150.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) inhibited by 20427536
Envelope surface glycoprotein gp120 downregulates 12775420
Pr55(Gag) interacts with 23027949
23051622

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)
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