Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004254
UniProt IDO43633
Primary gene name(s)CHMP2A
Synonym gene name(s)BC2, CHMP2
Protein nameCharged multivesicular body protein 2a
Protein functionProbable core component of the endosomal sorting required for transport complex III, ESCRT-III which is involved in multivesicular bodies, MVBs formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles, ILVs that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis, PubMed:21310966. Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase, PubMed:26040712. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. {ECO:0000269|PubMed:21310966, ECO:0000269|PubMed:26040712, ECO:0000305}.; FUNCTION:, Microbial infection The ESCRT machinery functions in topologically equivalent membrane fission events, such as the budding of enveloped viruses, HIV-1 and other lentiviruses. Involved in HIV-1 p6- and p9-dependent virus release. {ECO:0000269|PubMed:14505570, ECO:0000269|PubMed:14519844}.
Subcellular locationLate endosome membrane {ECO:0000269|PubMed:16730941, ECO:0000269|PubMed:17853893};
Peripheral membrane protein {ECO:0000269|PubMed:16730941, ECO:0000269|PubMed:17853893};
Cytoplasmic side {ECO:0000269|PubMed:16730941, ECO:0000269|PubMed:17853893}. Note=Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O43633
Gene Ontology
(Biological Process)
Complete annatation
autophagy [GO:0006914];
cell separation after cytokinesis [GO:0000920];
endosomal transport [GO:0016197];
ESCRT III complex disassembly [GO:1904903];
establishment of protein localization [GO:0045184];
exit from mitosis [GO:0010458];
membrane invagination [GO:0010324];
mitotic metaphase plate congression [GO:0007080];
multivesicular body assembly [GO:0036258];
negative regulation of cell death [GO:0060548];
negative regulation of centriole elongation [GO:1903723];
nuclear envelope reassembly [GO:0031468];
nucleus organization [GO:0006997];
positive regulation of exosomal secretion [GO:1903543];
positive regulation of viral release from host cell [GO:1902188];
protein heterooligomerization [GO:0051291];
protein homooligomerization [GO:0051260];
protein polymerization [GO:0051258];
protein transport [GO:0015031];
regulation of centrosome duplication [GO:0010824];
regulation of mitotic spindle assembly [GO:1901673];
regulation of viral process [GO:0050792];
vacuolar transport [GO:0007034];
viral budding via host ESCRT complex [GO:0039702];
viral life cycle [GO:0019058]
Gene Ontology
(Molecular Function)
Complete annatation
phosphatidylcholine binding [GO:0031210];
protein domain specific binding [GO:0019904]
Gene Ontology
(Cellular Component)
Complete annatation
cytosol [GO:0005829];
ESCRT III complex [GO:0000815];
extracellular exosome [GO:0070062];
late endosome membrane [GO:0031902];
membrane [GO:0016020];
membrane coat [GO:0030117];
nuclear envelope [GO:0005635]
Protein-protein interaction118091
Phylogenetic treeO43633
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.332155721920940.3108653970970590.430165558671216
AZA vs. DISU-0.1872043943228760.4608442395551870.924263941482596
AZA vs. IL70.133056126367790.4903790677822340.999311006273513
AZA vs. SAHA-0.3138807948835390.199741269274510.567493582512363
DISU vs. CD3-0.5310440653019770.1449153671642680.249224671378686
DISU vs. IL70.3103650175618820.220340188457950.602331495273807
DISU vs. SAHA-0.1244689485290150.6699063954722860.899355005002924
DMSO vs. AZA-0.02786895172529980.8685600694681431
DMSO vs. CD3-0.3689761738667790.2490114775227430.354537180689927
DMSO vs. DISU0.1581082105189210.5187067250520490.914196078390533
DMSO vs. IL70.1678698687943790.352207780497620.817731755891209
DMSO vs. SAHA-0.2922005152750220.2167586883602980.56014578056894
HIV vs. Mock in Activation-0.02874609099520480.9632171124338190.999983755607037
HIV vs. Mock in Latency-0.05529396118106140.7391497147359940.999834320637052
IL7 vs. CD3-0.1931821430140180.5475578289163140.67116688259377
SAHA vs. CD3-0.669194212074950.0594089743432240.114166442661615
SAHA vs. IL7-0.4487032405766960.0666442105650190.222206332682393
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.155162 0.345926
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.967 1.227 1.49 1.697 1.105
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
202121_s_at 1.51 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) co-localizes with 24436186
Pr55(Gag) regulated by 24107264
HIV-1 virus replication enhanced by expression of human gene 19460752
Pr55(Gag) interacts with 23027949

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)