Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004176
UniProt IDO00311
Primary gene name(s)CDC7
Synonym gene name(s)CDC7L1
Protein nameCell division cycle 7-related protein kinase
Protein functionSeems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3. {ECO:0000269|PubMed:12065429}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O00311
Gene Ontology
(Biological Process)
Complete annatation
cell cycle phase transition [GO:0044770];
cell division [GO:0051301];
DNA replication [GO:0006260];
DNA replication initiation [GO:0006270];
double-strand break repair via break-induced replication [GO:0000727];
G1/S transition of mitotic cell cycle [GO:0000082];
peptidyl-serine phosphorylation [GO:0018105];
phagocytosis [GO:0006909];
positive regulation of cell proliferation [GO:0008284];
positive regulation of G2/M transition of mitotic cell cycle [GO:0010971];
positive regulation of nuclear cell cycle DNA replication [GO:0010571];
regulation of cell shape [GO:0008360]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
kinase activity [GO:0016301];
metal ion binding [GO:0046872];
protein kinase activity [GO:0004672];
protein serine/threonine kinase activity [GO:0004674]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
intercellular bridge [GO:0045171];
microtubule cytoskeleton [GO:0015630];
nucleoplasm [GO:0005654];
nucleus [GO:0005634]
Protein-protein interaction113914
Phylogenetic treeO00311
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD32.068112375027740.01050952553926310.0271697373804071
AZA vs. DISU-0.07341289180311660.7821702497860720.977494719316423
AZA vs. IL70.6843178903072730.07046446789295270.750234020408019
AZA vs. SAHA1.132597054876699.82791835901775e-060.000664332023963379
DISU vs. CD3-2.155378484063360.008507336152828170.0252788458836452
DISU vs. IL70.7486521450955310.06912044652640650.348958717811647
DISU vs. SAHA1.207515430295746.53609034954039e-050.00279844936528191
DMSO vs. AZA-0.07942393894878790.672961914103181
DMSO vs. CD3-2.157355711767770.007626730672599110.0197213693070212
DMSO vs. DISU-0.006914020298066910.9784871422580630.996194243487317
DMSO vs. IL70.7688775883649130.03436514579839470.379527159641611
DMSO vs. SAHA1.203833522354431.15619551510626e-069.62708431390413e-05
HIV vs. Mock in Activation-0.3161692018283890.8414405824569830.999983755607037
HIV vs. Mock in Latency-0.2290434147718150.2085184507192990.999834320637052
IL7 vs. CD3-1.374451611758890.09417202158537610.180914463593726
SAHA vs. CD3-0.9593214867616170.234519882651510.341734155053012
SAHA vs. IL70.4455158658711980.2256113345053740.465999624052345
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.587073 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.59 0.785 1 1.068 0.807
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4F99 X-ray 2.3Å A=37-574.
4F9A X-ray 2.1Å A/C=37-574.
4F9B X-ray 2.5Å A/C=37-574.
4F9C X-ray 2.0Å A=37-574.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)