Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004175
UniProt IDQ99741
Primary gene name(s)CDC6
Synonym gene name(s)CDC18L
Protein nameCell division control protein 6 homolog
Protein functionInvolved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
Subcellular locationNucleus {ECO:0000269|PubMed:9566895}. Cytoplasm {ECO:0000269|PubMed:9566895}. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99741
Gene Ontology
(Biological Process)
Complete annatation
cell division [GO:0051301];
cellular response to angiotensin [GO:1904385];
cellular response to vasopressin [GO:1904117];
DNA replication [GO:0006260];
DNA replication checkpoint [GO:0000076];
DNA replication initiation [GO:0006270];
G1/S transition of mitotic cell cycle [GO:0000082];
mitotic nuclear division [GO:0007067];
negative regulation of cell proliferation [GO:0008285];
negative regulation of DNA replication [GO:0008156];
positive regulation of chromosome segregation [GO:0051984];
positive regulation of cyclin-dependent protein serine/threonine kinase activity [GO:0045737];
positive regulation of cytokinesis [GO:0032467];
positive regulation of fibroblast proliferation [GO:0048146];
regulation of cyclin-dependent protein serine/threonine kinase activity [GO:0000079];
regulation of mitotic metaphase/anaphase transition [GO:0030071];
regulation of transcription involved in G1/S transition of mitotic cell cycle [GO:0000083];
traversing start control point of mitotic cell cycle [GO:0007089]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
kinase binding [GO:0019900];
nucleotide binding [GO:0000166]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
Golgi apparatus [GO:0005794];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
spindle midzone [GO:0051233];
spindle pole [GO:0000922]
Protein-protein interaction107426
Phylogenetic treeQ99741
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD33.443707133657910.0006541418768594150.00250552131334819
AZA vs. DISU0.4943445390663660.5158077234394620.938437432475966
AZA vs. IL70.9991417625667570.04812344052979110.644768550316037
AZA vs. SAHA-0.8513076971819470.07713691925832240.345238100029677
DISU vs. CD3-2.964669967123280.008484705144630290.0252314944466674
DISU vs. IL70.4966932843559850.5383418793856590.848177856600662
DISU vs. SAHA-1.345955483957110.0951925775292130.394025634700019
DMSO vs. AZA-0.02000016229053960.9534918570836521
DMSO vs. CD3-3.476978909335220.0005494278186168880.00203069923637473
DMSO vs. DISU-0.515853270106050.492826792735240.906111920065799
DMSO vs. IL71.02594323946580.03243273058282050.368442662800316
DMSO vs. SAHA-0.8378273259374850.06293909130998980.289230225240485
HIV vs. Mock in Activation0.6310453714669480.7357559342175820.999983755607037
HIV vs. Mock in Latency-0.2439472695869180.275346575705460.999834320637052
IL7 vs. CD3-2.436448069319220.01623770630311360.0443550103491902
SAHA vs. CD3-4.323081062027315.91327051342461e-050.000301057387288205
SAHA vs. IL7-1.855044899366950.001700521288156120.0174973774045497
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.780189 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.388 0.738 4.42 5.436 0.702
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2CCH X-ray 1.7Å E/F=89-100.
2CCI X-ray 2.7Å F/I=71-100.
4I5L X-ray 2.4Å B/E=70-90.
4I5N X-ray 2.8Å B/E=70-90.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat interacts with 15050687

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)