Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004167
UniProt IDP30260
Primary gene name(s)CDC27
Synonym gene name(s)ANAPC3, D0S1430E, D17S978E
Protein nameCell division cycle protein 27 homolog
Protein functionComponent of the anaphase promoting complex/cyclosome, APC/C, a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. {ECO:0000269|PubMed:18485873}.
Subcellular locationNucleus.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P30260
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
cell proliferation [GO:0008283];
metaphase/anaphase transition of mitotic cell cycle [GO:0007091];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein K11-linked ubiquitination [GO:0070979];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051439]
Gene Ontology
(Molecular Function)
Complete annatation
protein phosphatase binding [GO:0019903]
Gene Ontology
(Cellular Component)
Complete annatation
anaphase-promoting complex [GO:0005680];
centrosome [GO:0005813];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
spindle [GO:0005819]
Protein-protein interaction107431
Phylogenetic treeP30260
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6685469348934860.04240027222437150.0869701827661577
AZA vs. DISU0.277159200787350.274145383667340.846086444137247
AZA vs. IL70.1245685731755710.5177262315329960.999311006273513
AZA vs. SAHA-0.04991717494536290.8382350796368220.961440456451378
DISU vs. CD3-0.4043033283548110.2648220187289430.392997758078328
DISU vs. IL7-0.1612479830172980.5222169140662650.8389194351608
DISU vs. SAHA-0.3264706597397540.2657283288399390.643869190871467
DMSO vs. AZA-0.08696416814646480.6046632640841951
DMSO vs. CD3-0.7675614031973640.01717639186867430.0393707140139363
DMSO vs. DISU-0.3661664337429270.1344339890655190.625334218112252
DMSO vs. IL70.2188375406350780.2246233520037880.731490098375259
DMSO vs. SAHA0.02982301274429220.8995655118777050.975555582924909
HIV vs. Mock in Activation-0.04335383713431460.9446159966170630.999983755607037
HIV vs. Mock in Latency0.06668377441924780.6867835489164240.999834320637052
IL7 vs. CD3-0.5351009501729850.09663794143174880.184827266996669
SAHA vs. CD3-0.7441068541353490.03759516657913580.0784056407898666
SAHA vs. IL7-0.1785672853091650.4653100574892860.70039421237575
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.232074 0.0916592
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.003 1.193 1.142 1.066 1.153
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3T1N X-ray 2.6Å C/D=821-824.
4RG6 X-ray 3.3Å A/B=1-824.
4RG7 X-ray 4.2Å A/B=1-824.
4RG9 X-ray 3.2Å A/B=1-824.
4UI9 EM 3.6Å F/H=1-824.
5A31 EM 4.3Å F/H=1-824.
5G04 EM 4.0Å F/H=1-824.
5G05 EM 3.4Å F/H=1-824.
5KHR EM 6.1Å F/H=1-824.
5L9U EM 6.4Å F/H=1-824.
5LCW EM 4.0Å F/H=1-824.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04114 Oocyte meiosis - Homo sapiens (human)
hsa04120 Ubiquitin mediated proteolysis - Homo sapiens (human)
hsa04914 Progesterone-mediated oocyte maturation - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)