Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0004135
UniProt IDP30281
Primary gene name(s)CCND3
Synonym gene name(s)unknown
Protein nameG1/S-specific cyclin-D3
Protein functionRegulatory component of the cyclin D3-CDK4, DC complex that phosphorylates and inhibits members of the retinoblastoma, RB protein family including RB1 and regulates the cell-cycle during G(1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1 phase. Hypophosphorylates RB1 in early G(1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. {ECO:0000269|PubMed:15358120}.
Subcellular locationNucleus {ECO:0000269|PubMed:15358120}. Cytoplasm {ECO:0000269|PubMed:15358120}. Membrane {ECO:0000269|PubMed:15358120}. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P30281
Gene Ontology
(Biological Process)
Complete annatation
cell cycle [GO:0007049];
cell division [GO:0051301];
positive regulation of cyclin-dependent protein serine/threonine kinase activity [GO:0045737];
positive regulation of protein phosphorylation [GO:0001934];
signal transduction [GO:0007165];
T cell proliferation [GO:0042098]
Gene Ontology
(Molecular Function)
Complete annatation
cyclin-dependent protein serine/threonine kinase activity [GO:0004693];
protein kinase binding [GO:0019901]
Gene Ontology
(Cellular Component)
Complete annatation
cyclin-dependent protein kinase holoenzyme complex [GO:0000307];
cytoplasm [GO:0005737];
focal adhesion [GO:0005925];
nucleoplasm [GO:0005654];
plasma membrane [GO:0005886]
Protein-protein interaction107336
Phylogenetic treeP30281
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.683100527338540.0007450116846308140.00280369940343461
AZA vs. DISU-0.2035672725532410.420334574929680.911468976967245
AZA vs. IL70.1798798957604160.3486893232189340.999311006273513
AZA vs. SAHA-0.4505441203495040.06469766117519150.312380699729288
DISU vs. CD31.468170471894060.003033011996215310.0106572547391491
DISU vs. IL70.3737087292068240.1376422799005160.485542188041558
DISU vs. SAHA-0.2443455422637190.4014344443194220.760893072002477
DMSO vs. AZA-0.02427096469157950.8843818585465341
DMSO vs. CD31.647516353849440.0006861917959154470.0024595478004916
DMSO vs. DISU0.1775850260003820.4659155036674460.895465711040481
DMSO vs. IL70.211450221029720.2385724098059760.744318806053491
DMSO vs. SAHA-0.4310046068828050.06746086633475850.302050717923691
HIV vs. Mock in Activation-0.1438985735367750.8906640268151430.999983755607037
HIV vs. Mock in Latency-0.04309767701726980.8512858656563930.999834320637052
IL7 vs. CD31.868586863422920.0001354406242076720.000761468306110497
SAHA vs. CD31.210169413285590.01610882813826320.0383689015400073
SAHA vs. IL7-0.6322837096981130.009673194480270380.0610099303747262
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.3315 0.04151

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.219631 0.113832
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 0.915 0.801 0.909 1.003
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
3G33 X-ray 3.0Å B/D=1-292.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
matrix upregulates 25704763
Tat interacts with 15050687

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa04115 p53 signaling pathway - Homo sapiens (human)
hsa04151 PI3K-Akt signaling pathway - Homo sapiens (human)
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa04510 Focal adhesion - Homo sapiens (human)
hsa04630 Jak-STAT signaling pathway - Homo sapiens (human)
hsa05162 Measles - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
Menu