Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003977
UniProt IDO60566
Primary gene name(s)BUB1B
Synonym gene name(s)BUBR1, MAD3L, SSK1
Protein nameMitotic checkpoint serine/threonine-protein kinase BUB1 beta
Protein functionEssential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome, APC/C by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. {ECO:0000269|PubMed:10477750, ECO:0000269|PubMed:11702782, ECO:0000269|PubMed:14706340, ECO:0000269|PubMed:15020684, ECO:0000269|PubMed:19411850, ECO:0000269|PubMed:19503101}.
Subcellular locationCytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O60566
Gene Ontology
(Biological Process)
Complete annatation
anaphase-promoting complex-dependent catabolic process [GO:0031145];
apoptotic process [GO:0006915];
cell division [GO:0051301];
cell proliferation [GO:0008283];
metaphase/anaphase transition of mitotic cell cycle [GO:0007091];
mitotic cell cycle checkpoint [GO:0007093];
mitotic nuclear division [GO:0007067];
mitotic spindle assembly checkpoint [GO:0007094];
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [GO:0051436];
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition [GO:0051437];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein localization to kinetochore [GO:0034501];
protein ubiquitination involved in ubiquitin-dependent protein catabolic process [GO:0042787];
sister chromatid cohesion [GO:0007062]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
protein kinase activity [GO:0004672];
protein serine/threonine kinase activity [GO:0004674]
Gene Ontology
(Cellular Component)
Complete annatation
anaphase-promoting complex [GO:0005680];
condensed chromosome kinetochore [GO:0000777];
condensed chromosome outer kinetochore [GO:0000940];
condensed nuclear chromosome kinetochore [GO:0000778];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
kinetochore [GO:0000776];
microtubule organizing center [GO:0005815];
perinuclear region of cytoplasm [GO:0048471];
spindle midzone [GO:0051233]
Protein-protein interaction107166
Phylogenetic treeO60566
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.1025418143624340.9287464338137350.954131705435254
AZA vs. DISU0.7002825001821230.07130662405107720.579447259934905
AZA vs. IL70.03696722403129540.914848930911610.999311006273513
AZA vs. SAHA-0.385386894041720.2278802652566950.597714828341644
DISU vs. CD30.5848385440266520.596514282513440.707630148312552
DISU vs. IL7-0.6719766697848350.1342952131434630.478950468966487
DISU vs. SAHA-1.085039464384280.01308317391929350.123854594307583
DMSO vs. AZA0.003974632151608410.9864665262052721
DMSO vs. CD3-0.1126199255483630.9212466917192090.945922942390259
DMSO vs. DISU-0.6993014922635560.06216590282514640.476879729610982
DMSO vs. IL70.04079315857632570.9020330159224890.979228917441583
DMSO vs. SAHA-0.3969038417246960.1891782476943720.524239041305403
HIV vs. Mock in Activation-0.02445751606483560.9904207693451880.999983755607037
HIV vs. Mock in Latency0.04758881862643410.9096148299756160.999834320637052
IL7 vs. CD3-0.05646119623400520.9597171015930680.975976353853607
SAHA vs. CD3-0.5149278038290060.6608648792671170.747521859232564
SAHA vs. IL7-0.4274340387970030.2805152714851910.527769246426332
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.561248 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.848 1.293 1.443 1.334 1.27
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2WVI X-ray 1.8Å A=57-220.
3SI5 X-ray 2.2Å A/B=57-220.
4GGD X-ray 2.4Å C/D=20-42.
5JJA X-ray 2.3Å C/D=647-720.
5LCW EM 4.0Å S=1-560.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat activates 24488929

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04110 Cell cycle - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)