Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003909
UniProt IDP12694
Primary gene name(s)BCKDHA
Synonym gene name(s)unknown
Protein name2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial
Protein functionThe branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase, E1, lipoamide acyltransferase, E2 and lipoamide dehydrogenase, E3.
Subcellular locationMitochondrion matrix.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P12694
Gene Ontology
(Biological Process)
Complete annatation
branched-chain amino acid catabolic process [GO:0009083];
glyoxylate metabolic process [GO:0046487]
Gene Ontology
(Molecular Function)
Complete annatation
3-methyl-2-oxobutanoate dehydrogenase, 2-methylpropanoyl-transferring activity [GO:0003863];
alpha-ketoacid dehydrogenase activity [GO:0003826];
carboxy-lyase activity [GO:0016831];
metal ion binding [GO:0046872]
Gene Ontology
(Cellular Component)
Complete annatation
mitochondrial alpha-ketoglutarate dehydrogenase complex [GO:0005947];
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739]
Protein-protein interaction107065
Phylogenetic treeP12694
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.0009914348765705580.9976019894506270.998146590836428
AZA vs. DISU-0.02252977351985680.9303259631900220.995042856396371
AZA vs. IL7-0.2061837845550710.2994409419689230.999311006273513
AZA vs. SAHA-0.4030679771771370.1067521093655290.415809120562268
DISU vs. CD3-0.0335253024606740.926955735215570.952288608508111
DISU vs. IL7-0.1935585234881470.44898073703740.796159688909002
DISU vs. SAHA-0.3782534869461120.2002279831900530.571305553702629
DMSO vs. AZA0.06703171362714490.6998112961794871
DMSO vs. CD30.05421190581958860.8663564747608050.907941585549324
DMSO vs. DISU0.08698769479325870.7253998819211250.964511754063116
DMSO vs. IL7-0.2654339763814250.1523878253973750.654913997838307
DMSO vs. SAHA-0.4744852227936430.04878343024617560.249056597981068
HIV vs. Mock in Activation0.03500635674706940.9553855465319290.999983755607037
HIV vs. Mock in Latency0.1971523703529650.2470540633019190.999834320637052
IL7 vs. CD3-0.2004385136231440.5374392375334980.66253550563036
SAHA vs. CD3-0.4253292810677040.2338575911256640.341098906142447
SAHA vs. IL7-0.1989176184490920.423489150256310.665788755746975
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.600075 0.0805229
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 0.915 0.998 1.043 0.875
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1DTW X-ray 2.7Å A=46-445.
1OLS X-ray 1.8Å A=46-445.
1OLU X-ray 1.9Å A=46-445.
1OLX X-ray 2.2Å A=46-445.
1U5B X-ray 1.8Å A=46-445.
1V11 X-ray 1.9Å A=46-445.
1V16 X-ray 1.9Å A=46-445.
1V1M X-ray 2.0Å A=46-445.
1V1R X-ray 1.8Å A=46-445.
1WCI X-ray 1.8Å A=46-445.
1X7W X-ray 1.7Å A=46-445.
1X7X X-ray 2.1Å A=46-445.
1X7Y X-ray 1.5Å A=46-445.
1X7Z X-ray 1.7Å A=46-445.
1X80 X-ray 2.0Å A=46-445.
2BEU X-ray 1.8Å A=46-445.
2BEV X-ray 1.8Å A=46-445.
2BEW X-ray 1.7Å A=46-445.
2BFB X-ray 1.7Å A=46-445.
2BFC X-ray 1.6Å A=46-445.
2BFD X-ray 1.3Å A=46-445.
2BFE X-ray 1.6Å A=46-445.
2BFF X-ray 1.4Å A=46-445.
2J9F X-ray 1.8Å A/C=46-445.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00280 Valine# leucine and isoleucine degradation - Homo sapiens (human)
hsa00640 Propanoate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
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