Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003894
UniProt IDQ99933
Primary gene name(s)BAG1
Synonym gene name(s)HAP
Protein nameBAG family molecular chaperone regulator 1
Protein functionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. {ECO:0000269|PubMed:12724406, ECO:0000269|PubMed:9305631, ECO:0000269|PubMed:9873016}.
Subcellular locationIsoform 1: Nucleus. Cytoplasm. Note=Isoform 1 localizes predominantly to the nucleus.;
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. Nucleus. Note=Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock.;
SUBCELLULAR LOCATION: Isoform 4: Cytoplasm. Nucleus. Note=Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes, what appears to be the nuclear envelope and perinuclear membranes as well as punctate cytosolic structures suggestive of mitochondria.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99933
Gene Ontology
(Biological Process)
Complete annatation
apoptotic process [GO:0006915];
cell surface receptor signaling pathway [GO:0007166];
chaperone cofactor-dependent protein refolding [GO:0070389];
negative regulation of apoptotic process [GO:0043066];
regulation of cellular response to heat [GO:1900034]
Gene Ontology
(Molecular Function)
Complete annatation
adenyl-nucleotide exchange factor activity [GO:0000774];
receptor signaling protein activity [GO:0005057]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleus [GO:0005634]
Protein-protein interaction107049
Phylogenetic treeQ99933
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.9109560940551950.006534232585417410.0181601897881559
AZA vs. DISU0.3445951049884160.1757081423950130.76025598363942
AZA vs. IL70.1274081689191870.5097838219826610.999311006273513
AZA vs. SAHA0.07832083334631120.7493560856388230.931381401296142
DISU vs. CD3-0.5792858171269720.1203690100451350.215639181035951
DISU vs. IL7-0.2262348230702090.3710405085295760.744446406382166
DISU vs. SAHA-0.264255077742360.3699615098349890.740969107537586
DMSO vs. AZA-0.1009024407897840.5503904550039811
DMSO vs. CD3-1.022763320768430.002318332178160460.00708862883377053
DMSO vs. DISU-0.4472746723212720.06843078189320980.48925254404225
DMSO vs. IL70.2356176398756910.1928054006283710.701742535690539
DMSO vs. SAHA0.1727960457067480.4662710799893280.792443844769281
HIV vs. Mock in Activation-0.07180737880589090.9235906574785540.999983755607037
HIV vs. Mock in Latency0.02792904061112650.8666637629763530.999834320637052
IL7 vs. CD3-0.7764759985080590.01782019243876120.0477348072915315
SAHA vs. CD3-0.8571852710144630.01832827595081980.042796268760281
SAHA vs. IL7-0.0520665037983710.8316203354469290.927344646880235
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.286 0.04905

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.279579 0.0431012
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.996 1.022 1.036 1.025 1.172
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB07045 (2R,3R,4S,5R)-2-[6-amino-8-[(3,4-dichlorophenyl)methylamino]purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1HX1 X-ray 1.9Å B=222-334.
1WXV NMR - A=144-222.
3FZF X-ray 2.2Å B=222-334.
3FZH X-ray 2.0Å B=222-334.
3FZK X-ray 2.1Å B=222-334.
3FZL X-ray 2.2Å B=222-334.
3FZM X-ray 2.3Å B=222-334.
3LDQ X-ray 1.9Å B=222-334.
3M3Z X-ray 2.1Å B=222-334.
5AQF X-ray 1.8Å B/D=222-334.
5AQG X-ray 2.2Å B/D/F=222-334.
5AQH X-ray 2.0Å B=222-334.
5AQI X-ray 1.9Å B/D=222-334.
5AQJ X-ray 1.9Å B/D/F=222-334.
5AQK X-ray 2.0Å B=222-334.
5AQL X-ray 1.6Å B/D=222-334.
5AQM X-ray 1.6Å B/D=222-334.
5AQN X-ray 2.4Å B/D/F=222-334.
5AQO X-ray 2.1Å B/D/F=222-334.
5AQP X-ray 2.0Å B/D/F=222-334.
5AQQ X-ray 2.7Å B/D/F=222-334.
5AQR X-ray 1.9Å B/D/F=222-334.
5AQS X-ray 2.0Å B/D=222-334.
5AQT X-ray 1.9Å B=222-334.
5AQU X-ray 1.9Å B=222-334.
5AQV X-ray 1.7Å B=222-334.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Tat upregulates 11994280

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04141 Protein processing in endoplasmic reticulum - Homo sapiens (human)