Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003890
UniProt IDO94766
Primary gene name(s)B3GAT3
Synonym gene name(s)unknown
Protein nameGalactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
Protein functionGlycosaminoglycans biosynthesis, PubMed:25893793. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid, UDP-GlcUA to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid, UDP-GlcUA during completion of linkage region formation, PubMed:24425863. {ECO:0000269|PubMed:24425863, ECO:0000269|PubMed:25893793}.
Subcellular locationGolgi apparatus membrane {ECO:0000269|PubMed:21763480};
Single-pass type II membrane protein {ECO:0000269|PubMed:21763480}. Golgi apparatus, cis-Golgi network {ECO:0000269|PubMed:21763480, ECO:0000269|PubMed:25893793}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O94766
Gene Ontology
(Biological Process)
Complete annatation
carbohydrate metabolic process [GO:0005975];
chondroitin sulfate metabolic process [GO:0030204];
chondroitin sulfate proteoglycan biosynthetic process [GO:0050650];
dermatan sulfate proteoglycan biosynthetic process [GO:0050651];
glycosaminoglycan biosynthetic process [GO:0006024];
glycosaminoglycan metabolic process [GO:0030203];
heparan sulfate proteoglycan biosynthetic process [GO:0015012];
positive regulation of catalytic activity [GO:0043085];
positive regulation of intracellular protein transport [GO:0090316];
protein glycosylation [GO:0006486]
Gene Ontology
(Molecular Function)
Complete annatation
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity [GO:0015018];
glucuronosyltransferase activity [GO:0015020];
metal ion binding [GO:0046872];
protein phosphatase activator activity [GO:0072542]
Gene Ontology
(Cellular Component)
Complete annatation
cis-Golgi network [GO:0005801];
extracellular exosome [GO:0070062];
Golgi apparatus [GO:0005794];
Golgi membrane [GO:0000139];
integral component of membrane [GO:0016021];
membrane [GO:0016020]
Protein-protein interaction117620
Phylogenetic treeO94766
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.326607884114680.319251315378230.438908676947979
AZA vs. DISU-0.1776870291038070.483459936606750.931429104131977
AZA vs. IL7-0.05462830034888530.777251918179050.999311006273513
AZA vs. SAHA-0.5457345659585220.02618888310855650.183443877313374
DISU vs. CD30.1371300562556290.7053326671653470.79374278647633
DISU vs. IL70.1132825531187550.654016284144830.900798987354818
DISU vs. SAHA-0.3652895056218880.2118339218392370.586339658063564
DMSO vs. AZA-0.009449667068665320.9552749937098531
DMSO vs. CD30.3044021414647380.3417303679570010.455791765672617
DMSO vs. DISU0.1660394501917770.4973359518004820.907526896115356
DMSO vs. IL7-0.03749056839003870.8355349581012110.963494783989913
DMSO vs. SAHA-0.5404762679108480.02264575353670660.154083138398061
HIV vs. Mock in Activation-0.1466536489136280.8142713843825420.999983755607037
HIV vs. Mock in Latency0.03781223950902740.819679700891370.999834320637052
IL7 vs. CD30.2772374541015610.388881058924940.525849436886653
SAHA vs. CD3-0.2415500484515010.4946876776038080.604177267322262
SAHA vs. IL7-0.4929416705644140.04405644485453160.16859782933161
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0388898 0.843274
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.993 1.012 1.191 1.109 0.862
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB03041 UDP-alpha-D-glucuronic acid experimental unknown unknown
DB03435 Uridine-5&,39;-Diphosphate experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1FGG X-ray 2.3Å A/B=76-335.
1KWS X-ray 2.1Å A/B=76-335.
3CU0 X-ray 1.9Å A/B=76-335.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00532 Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate - Homo sapiens (human)
hsa00534 Glycosaminoglycan biosynthesis - heparan sulfate / heparin - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)