Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003864
UniProt IDP61421
Primary gene name(s)ATP6V0D1
Synonym gene name(s)ATP6D, VPATPD
Protein nameV-type proton ATPase subunit d 1
Protein functionSubunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis, By similarity. May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium, By similarity. {ECO:0000250}.
Subcellular locationMembrane {ECO:0000305|PubMed:21844891};
Peripheral membrane protein {ECO:0000305|PubMed:21844891};
Cytoplasmic side {ECO:0000305|PubMed:21844891}. Note=Localizes to centrosome and the base of the cilium.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P61421
Gene Ontology
(Biological Process)
Complete annatation
ATP hydrolysis coupled proton transport [GO:0015991];
brain development [GO:0007420];
cilium assembly [GO:0060271];
insulin receptor signaling pathway [GO:0008286];
ion transmembrane transport [GO:0034220];
IRE1-mediated unfolded protein response [GO:0036498];
phagosome acidification [GO:0090383];
proton transport [GO:0015992];
regulation of macroautophagy [GO:0016241];
transferrin transport [GO:0033572];
vacuolar acidification [GO:0007035];
vacuolar transport [GO:0007034]
Gene Ontology
(Molecular Function)
Complete annatation
hydrogen-exporting ATPase activity, phosphorylative mechanism [GO:0008553]
Gene Ontology
(Cellular Component)
Complete annatation
apical plasma membrane [GO:0016324];
axon terminus [GO:0043679];
early endosome [GO:0005769];
endosome membrane [GO:0010008];
extracellular exosome [GO:0070062];
lysosomal membrane [GO:0005765];
membrane [GO:0016020];
phagocytic vesicle membrane [GO:0030670];
proton-transporting V-type ATPase, V0 domain [GO:0033179];
synaptic vesicle [GO:0008021];
vacuolar proton-transporting V-type ATPase complex [GO:0016471]
Protein-protein interaction114564
Phylogenetic treeP61421
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.2070639212536160.5282487229094320.643039841182536
AZA vs. DISU0.1852359131298880.4651000829806670.925522269888509
AZA vs. IL70.006248989348808420.9742262801967550.999311006273513
AZA vs. SAHA0.1906283550315870.4380356062797510.786533499590347
DISU vs. CD30.379906994601040.2956016165412130.426810226795234
DISU vs. IL7-0.1887437467924780.4547314476356920.79858767161091
DISU vs. SAHA0.00813308065947550.9778742378950020.995922620191181
DMSO vs. AZA0.04204791247170620.803398289496291
DMSO vs. CD30.2369131858562020.4596969357823180.57097323254684
DMSO vs. DISU-0.1453437997378270.5522218531625020.923211762416618
DMSO vs. IL7-0.02839132715874050.875268439870650.972762986031258
DMSO vs. SAHA0.143648004462310.5456464415205050.836650475140455
HIV vs. Mock in Activation-0.009810685869626550.9874448655730130.999983755607037
HIV vs. Mock in Latency0.01348118774959260.935528545484860.999834320637052
IL7 vs. CD30.2191015738986660.4958754509343450.626380398806309
SAHA vs. CD30.3743020138561990.2953526163236950.408307855058994
SAHA vs. IL70.1823692746369610.4585408532832710.695446707152835
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0212231 0.91743
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.002 1.058 1.057 1.23 1.004
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
212041_at 1.39 No upregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00190 Oxidative phosphorylation - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04142 Lysosome - Homo sapiens (human)
hsa04145 Phagosome - Homo sapiens (human)
hsa04721 Synaptic vesicle cycle - Homo sapiens (human)
hsa04966 Collecting duct acid secretion - Homo sapiens (human)
hsa05110 Vibrio cholerae infection - Homo sapiens (human)
hsa05120 Epithelial cell signaling in Helicobacter pylori infection - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)
hsa05203 Viral carcinogenesis - Homo sapiens (human)
hsa05323 Rheumatoid arthritis - Homo sapiens (human)