Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003861
UniProt IDQ15904
Primary gene name(s)ATP6AP1
Synonym gene name(s)ATP6IP1, ATP6S1, VATPS1, XAP3
Protein nameV-type proton ATPase subunit S1
Protein functionAccessory subunit of the proton-transporting vacuolar, V-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. {ECO:0000305|PubMed:27231034}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:27231034};
Single-pass membrane protein {ECO:0000305}. Endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000269|PubMed:27231034}. Note=Not detected in trans-Golgi network. {ECO:0000269|PubMed:27231034}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q15904
Gene Ontology
(Biological Process)
Complete annatation
ATP hydrolysis coupled proton transport [GO:0015991];
establishment of organelle localization [GO:0051656];
insulin receptor signaling pathway [GO:0008286];
ion transmembrane transport [GO:0034220];
pH reduction [GO:0045851];
positive regulation of bone resorption [GO:0045780];
positive regulation of ERK1 and ERK2 cascade [GO:0070374];
positive regulation of exocytosis [GO:0045921];
positive regulation of osteoblast differentiation [GO:0045669];
positive regulation of osteoclast development [GO:2001206];
proton transport [GO:0015992];
transferrin transport [GO:0033572]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
proton-transporting ATPase activity, rotational mechanism [GO:0046961];
proton-transporting ATP synthase activity, rotational mechanism [GO:0046933];
Rab GTPase binding [GO:0017137];
transporter activity [GO:0005215]
Gene Ontology
(Cellular Component)
Complete annatation
endosome membrane [GO:0010008];
extracellular exosome [GO:0070062];
integral component of membrane [GO:0016021];
proton-transporting two-sector ATPase complex [GO:0016469];
proton-transporting V-type ATPase, V1 domain [GO:0033180]
Protein-protein interaction107019
Phylogenetic treeQ15904
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.7811054129916550.01769009161050470.0420876181142111
AZA vs. DISU0.1507683612537090.5511117119507460.946928765276725
AZA vs. IL7-0.1456568322786740.4492792683759610.999311006273513
AZA vs. SAHA-0.3566794894538030.1444027063474150.4840396698868
DISU vs. CD30.9201557879115850.01175198687478020.0331740543352877
DISU vs. IL7-0.3064094368699820.2241043064630150.606852077089702
DISU vs. SAHA-0.5047090379601330.08389500326559880.368302241251337
DMSO vs. AZA0.02240821297030270.8937485757215231
DMSO vs. CD30.7931114607151790.01367441835582390.0324980458322271
DMSO vs. DISU-0.1299736552911180.5941135573402820.936830732355137
DMSO vs. IL7-0.1607985822911820.371775539681620.830194188375728
DMSO vs. SAHA-0.3842768089209720.10371282440880.381277032193345
HIV vs. Mock in Activation0.3271451456615390.5995059226702110.999983755607037
HIV vs. Mock in Latency0.1182187998269110.4742694617587940.999834320637052
IL7 vs. CD30.6402863386391780.04712181668077640.104450002189249
SAHA vs. CD30.4019832583131130.2558153022800490.366040315738734
SAHA vs. IL7-0.2128788162611890.3824828376664770.63286236471295
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.135427 0.373443
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.971 1.004 0.968 0.962 0.948
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef requires 25496667
HIV-1 virus replication enhanced by expression of human gene 25496667

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00190 Oxidative phosphorylation - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04142 Lysosome - Homo sapiens (human)
hsa04145 Phagosome - Homo sapiens (human)
hsa05110 Vibrio cholerae infection - Homo sapiens (human)
hsa05120 Epithelial cell signaling in Helicobacter pylori infection - Homo sapiens (human)
hsa05152 Tuberculosis - Homo sapiens (human)
hsa05161 Hepatitis B - Homo sapiens (human)
hsa05323 Rheumatoid arthritis - Homo sapiens (human)