Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003815
UniProt IDP00966
Primary gene name(s)ASS1
Synonym gene name(s)ASS
Protein nameArgininosuccinate synthase
Protein functionIs indirectly involved in the control of blood pressure. {ECO:0000250}.
Subcellular locationunknown
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P00966
Gene Ontology
(Biological Process)
Complete annatation
acute-phase response [GO:0006953];
aging [GO:0007568];
arginine biosynthetic process [GO:0006526];
argininosuccinate metabolic process [GO:0000053];
aspartate metabolic process [GO:0006531];
cellular response to amine stimulus [GO:0071418];
cellular response to amino acid stimulus [GO:0071230];
cellular response to ammonium ion [GO:0071242];
cellular response to cAMP [GO:0071320];
cellular response to dexamethasone stimulus [GO:0071549];
cellular response to glucagon stimulus [GO:0071377];
cellular response to interferon-gamma [GO:0071346];
cellular response to laminar fluid shear stress [GO:0071499];
cellular response to lipopolysaccharide [GO:0071222];
cellular response to oleic acid [GO:0071400];
cellular response to tumor necrosis factor [GO:0071356];
citrulline metabolic process [GO:0000052];
diaphragm development [GO:0060539];
kidney development [GO:0001822];
liver development [GO:0001889];
midgut development [GO:0007494];
negative regulation of leukocyte cell-cell adhesion [GO:1903038];
positive regulation of nitric oxide biosynthetic process [GO:0045429];
response to drug [GO:0042493];
response to estradiol [GO:0032355];
response to fatty acid [GO:0070542];
response to growth hormone [GO:0060416];
response to mycotoxin [GO:0010046];
response to nutrient [GO:0007584];
response to zinc ion [GO:0010043];
urea cycle [GO:0000050]
Gene Ontology
(Molecular Function)
Complete annatation
amino acid binding [GO:0016597];
argininosuccinate synthase activity [GO:0004055];
ATP binding [GO:0005524];
identical protein binding [GO:0042802];
poly(A RNA binding [GO:0044822];
toxic substance binding [GO:0015643]
Gene Ontology
(Cellular Component)
Complete annatation
cell body fiber [GO:0070852];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
endoplasmic reticulum [GO:0005783];
extracellular exosome [GO:0070062];
lysosome [GO:0005764];
mitochondrial outer membrane [GO:0005741];
nucleus [GO:0005634];
perikaryon [GO:0043204]
Protein-protein interaction106937
Phylogenetic treeP00966
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.733043389376740.009264790598603630.0244561985798845
AZA vs. DISUunknownunknownunknown
AZA vs. IL70.6734735459058250.1725603547969590.941150532286992
AZA vs. SAHA0.1440561211427020.6780785321449570.90621672242426
DISU vs. CD3-2.101575971301520.003688029861435680.0126033466631788
DISU vs. IL71.018982952887490.07165685588592220.356006963027397
DISU vs. SAHA0.4969178786134260.2692701957572040.647616251796192
DMSO vs. AZA0.1639776876518140.707006005623741
DMSO vs. CD3-1.58448528232240.02004945268248020.0449271165642665
DMSO vs. DISU0.5161062530144310.3174100616591650.822006745975451
DMSO vs. IL70.5178254802680830.3216336419593570.799721223986312
DMSO vs. SAHA-0.02602563340098210.9479079693969520.987880038071194
HIV vs. Mock in Activation-0.1357095278464910.9157026038460950.999983755607037
HIV vs. Mock in Latency-0.07383986939230340.899432650232020.999834320637052
IL7 vs. CD3-1.056632173258340.1321103502541960.234878427622257
SAHA vs. CD3-1.609174918639530.01323776222766960.0324590102133063
SAHA vs. IL7-0.5346249839889590.240245309232290.482153879825261
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
NOTEST 1.06519 1
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.039 0.738 0.648 0.601 0.455
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00125 L-Arginine approved, nutraceutical unknown unknown
DB00128 L-Aspartic Acid approved, nutraceutical unknown unknown
DB00155 L-Citrulline approved, nutraceutical unknown unknown
DB00171 Adenosine triphosphate approved, nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2NZ2 X-ray 2.4Å A=1-412.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00220 Arginine biosynthesis - Homo sapiens (human)
hsa00250 Alanine# aspartate and glutamate metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01230 Biosynthesis of amino acids - Homo sapiens (human)