Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003797
UniProt IDP59998
Primary gene name(s)ARPC4
Synonym gene name(s)ARC20
Protein nameActin-related protein 2/3 complex subunit 4
Protein functionFunctions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor, NPF mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Subcellular locationCytoplasm, cytoskeleton {ECO:0000269|PubMed:9359840}. Cell projection {ECO:0000269|PubMed:9359840}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P59998
Gene Ontology
(Biological Process)
Complete annatation
actin filament polymerization [GO:0030041];
actin nucleation [GO:0045010];
Arp2/3 complex-mediated actin nucleation [GO:0034314];
ephrin receptor signaling pathway [GO:0048013];
Fc-gamma receptor signaling pathway involved in phagocytosis [GO:0038096]
Gene Ontology
(Molecular Function)
Complete annatation
enzyme binding [GO:0019899];
structural constituent of cytoskeleton [GO:0005200]
Gene Ontology
(Cellular Component)
Complete annatation
Arp2/3 protein complex [GO:0005885];
cell projection [GO:0042995];
cytosol [GO:0005829];
extracellular exosome [GO:0070062]
Protein-protein interaction115400;1529294
Phylogenetic treeP59998
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.6483119275771720.04912069170583480.0983588501913567
AZA vs. DISU-0.147462776125990.5597196746023880.950206522099159
AZA vs. IL70.1186213560851510.53684350762030.999311006273513
AZA vs. SAHA-0.3793384639261030.1201434486270490.441458624666131
DISU vs. CD3-0.8078366791015710.02753870733394830.066782251343619
DISU vs. IL70.2561332153426540.3095874925040360.693116214161995
DISU vs. SAHA-0.2292373794146240.4314148501815050.77924947232387
DMSO vs. AZA0.04321423943465350.7962058272218781
DMSO vs. CD3-0.6152016773267320.05561196428409050.105276984410634
DMSO vs. DISU0.1892068619456210.437991319437130.882432295026854
DMSO vs. IL70.0824600952040970.646102693657630.92438865873712
DMSO vs. SAHA-0.4279137032995350.06974949586531180.307907045162892
HIV vs. Mock in Activation-0.0334017743432590.9572360688586460.999983755607037
HIV vs. Mock in Latency0.03737603784265050.820803955004370.999834320637052
IL7 vs. CD3-0.5241876145977320.1037288426814830.195096686515847
SAHA vs. CD3-1.050294831190420.003339106329925510.0100640664783955
SAHA vs. IL7-0.4994904102405620.04059874213389640.159205369127563
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
-0.2882 0.04557

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.118777 0.547042
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.05 1.119 1.109 1.187 1.147
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB08235 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide experimental unknown unknown
DB08236 (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)
hsa04666 Fc gamma R-mediated phagocytosis - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa05100 Bacterial invasion of epithelial cells - Homo sapiens (human)
hsa05130 Pathogenic Escherichia coli infection - Homo sapiens (human)
hsa05131 Shigellosis - Homo sapiens (human)
hsa05132 Salmonella infection - Homo sapiens (human)