Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003723
UniProt IDP25054
Primary gene name(s)APC
Synonym gene name(s)DP2.5
Protein nameAdenomatous polyposis coli protein
Protein functionTumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor, HGF-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization. {ECO:0000269|PubMed:10947987, ECO:0000269|PubMed:17599059, ECO:0000269|PubMed:19151759, ECO:0000269|PubMed:19893577, ECO:0000269|PubMed:20937854}.
Subcellular locationCell junction, adherens junction {ECO:0000269|PubMed:16611247}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:19632184, ECO:0000269|PubMed:20937854}. Cell projection, lamellipodium {ECO:0000269|PubMed:19151759}. Cell projection, ruffle membrane {ECO:0000269|PubMed:19151759}. Cytoplasm {ECO:0000269|PubMed:10947987}. Cell membrane {ECO:0000269|PubMed:10947987, ECO:0000269|PubMed:16611247, ECO:0000269|PubMed:20937854}. Note=Associated with the microtubule network at the growing distal tip of microtubules, PubMed:19632184. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor, HGF treatment, PubMed:19151759. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane, PubMed:20937854. {ECO:0000269|PubMed:19151759, ECO:0000269|PubMed:19632184, ECO:0000269|PubMed:20937854}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P25054
Gene Ontology
(Biological Process)
Complete annatation
beta-catenin destruction complex assembly [GO:1904885];
beta-catenin destruction complex disassembly [GO:1904886];
bicellular tight junction assembly [GO:0070830];
canonical Wnt signaling pathway [GO:0060070];
cell adhesion [GO:0007155];
cell cycle arrest [GO:0007050];
cell fate specification [GO:0001708];
cell migration [GO:0016477];
cellular component disassembly involved in execution phase of apoptosis [GO:0006921];
cellular response to DNA damage stimulus [GO:0006974];
mitotic cytokinesis [GO:0000281];
mitotic spindle assembly checkpoint [GO:0007094];
negative regulation of canonical Wnt signaling pathway [GO:0090090];
negative regulation of cell proliferation [GO:0008285];
negative regulation of cyclin-dependent protein serine/threonine kinase activity [GO:0045736];
negative regulation of microtubule depolymerization [GO:0007026];
pattern specification process [GO:0007389];
positive regulation of apoptotic process [GO:0043065];
positive regulation of cell migration [GO:0030335];
positive regulation of protein catabolic process [GO:0045732];
positive regulation of pseudopodium assembly [GO:0031274];
proteasome-mediated ubiquitin-dependent protein catabolic process [GO:0043161];
protein complex assembly [GO:0006461];
regulation of attachment of spindle microtubules to kinetochore [GO:0051988];
regulation of cell differentiation [GO:0045595];
regulation of microtubule-based process [GO:0032886];
Wnt signaling pathway [GO:0016055]
Gene Ontology
(Molecular Function)
Complete annatation
beta-catenin binding [GO:0008013];
gamma-catenin binding [GO:0045295];
microtubule binding [GO:0008017];
microtubule plus-end binding [GO:0051010];
protein kinase binding [GO:0019901];
protein kinase regulator activity [GO:0019887]
Gene Ontology
(Cellular Component)
Complete annatation
adherens junction [GO:0005912];
axonal growth cone [GO:0044295];
beta-catenin destruction complex [GO:0030877];
bicellular tight junction [GO:0005923];
catenin complex [GO:0016342];
cell body fiber [GO:0070852];
cell cortex [GO:0005938];
centrosome [GO:0005813];
cytoplasm [GO:0005737];
cytosol [GO:0005829];
dendrite [GO:0030425];
kinetochore [GO:0000776];
lamellipodium [GO:0030027];
lateral plasma membrane [GO:0016328];
microtubule [GO:0005874];
neuronal cell body [GO:0043025];
neuron projection terminus [GO:0044306];
nuclear membrane [GO:0031965];
nucleoplasm [GO:0005654];
nucleus [GO:0005634];
plasma membrane [GO:0005886];
ruffle membrane [GO:0032587];
synapse [GO:0045202];
Wnt signalosome [GO:1990909]
Protein-protein interaction106821
Phylogenetic treeP25054
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.6812409613510160.03890550908654320.0808247874503624
AZA vs. DISU0.394445055860190.2109765157972480.801002006259735
AZA vs. IL7-0.07158328896128220.713462382645830.999311006273513
AZA vs. SAHA-0.4211877487833730.08575096705052740.366070564749227
DISU vs. CD31.062573835902770.003953233760354610.0133552321164468
DISU vs. IL7-0.4741862879433260.119748283525370.452955631917449
DISU vs. SAHA-0.8155665118207640.007049618885305310.0819158105014929
DMSO vs. AZA-0.05564959119060680.7406928828469251
DMSO vs. CD30.6129149196184230.05670883074438460.107044046505971
DMSO vs. DISU-0.4522391077529820.1141101689783680.590652778477021
DMSO vs. IL7-0.008557586115888440.9621505216443450.993151606293804
DMSO vs. SAHA-0.3734323773337710.1146231910697470.402250737914772
HIV vs. Mock in Activation0.2003322884890790.7500477559883730.999983755607037
HIV vs. Mock in Latency-0.04157069293577370.801914986590010.999834320637052
IL7 vs. CD30.6191035390105990.05514497291434230.119139407324191
SAHA vs. CD30.2336109888502410.5089038667604590.617237929342464
SAHA vs. IL7-0.3550213617588820.14614350171450.360669146176223
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.799889 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.249 1.555 1.305 1.804 1.382
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1DEB X-ray 2.4Å A/B=2-55.
1EMU X-ray 1.9Å B=2034-2049.
1JPP X-ray 3.1Å C/D=1021-1035.
1M5I X-ray 2.0Å A=126-250.
1T08 X-ray 2.1Å C=1484-1498.
1TH1 X-ray 2.5Å C/D=1362-1540.
1V18 X-ray 2.1Å B=1482-1528.
2RQU NMR - B=1578-1596.
3AU3 X-ray 2.1Å A=396-732.
3NMW X-ray 1.6Å A/B=407-751.
3NMX X-ray 2.3Å A/B/C=407-751.
3NMZ X-ray 3.0Å A/B=303-739.
3QHE X-ray 2.4Å A/C=396-732.
3RL7 X-ray 2.3Å G/H/I/J/K/L=2833-2843.
3RL8 X-ray 2.2Å F=2833-2843.
3T7U X-ray 2.9Å A/B=407-775.
4G69 X-ray 2.0Å B=2833-2843.
4YJE X-ray 1.9Å A=407-751.
4YJL X-ray 2.1Å A/B/C/D/E/F=407-751.
4YK6 X-ray 1.7Å A=407-751.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04310 Wnt signaling pathway - Homo sapiens (human)
hsa04390 Hippo signaling pathway - Homo sapiens (human)
hsa04550 Signaling pathways regulating pluripotency of stem cells - Homo sapiens (human)
hsa04810 Regulation of actin cytoskeleton - Homo sapiens (human)
hsa05166 HTLV-I infection - Homo sapiens (human)
hsa05200 Pathways in cancer - Homo sapiens (human)
hsa05206 MicroRNAs in cancer - Homo sapiens (human)
hsa05210 Colorectal cancer - Homo sapiens (human)
hsa05213 Endometrial cancer - Homo sapiens (human)
hsa05217 Basal cell carcinoma - Homo sapiens (human)
hsa05224 Breast cancer - Homo sapiens (human)