Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003715
UniProt IDO14617
Primary gene name(s)AP3D1
Synonym gene name(s)unknown
Protein nameAP-3 complex subunit delta-1
Protein functionPart of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Involved in process of CD8+ T-cell and NK cell degranulation, PubMed:26744459. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals, By similarity. {ECO:0000250|UniProtKB:O54774, ECO:0000269|PubMed:26744459}.
Subcellular locationCytoplasm {ECO:0000250}. Golgi apparatus membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250};
Cytoplasmic side {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O14617
Gene Ontology
(Biological Process)
Complete annatation
anterograde axonal transport [GO:0008089];
anterograde synaptic vesicle transport [GO:0048490];
antigen processing and presentation, exogenous lipid antigen via MHC class Ib [GO:0048007];
endosome to melanosome transport [GO:0035646];
eye pigment biosynthetic process [GO:0006726];
intracellular protein transport [GO:0006886];
melanosome organization [GO:0032438];
positive regulation of NK T cell differentiation [GO:0051138];
protein localization to membrane [GO:0072657];
protein localization to organelle [GO:0033365];
regulation of sequestering of zinc ion [GO:0061088];
synaptic vesicle membrane organization [GO:0048499]
Gene Ontology
(Molecular Function)
Complete annatation
transporter activity [GO:0005215]
Gene Ontology
(Cellular Component)
Complete annatation
axon cytoplasm [GO:1904115];
endosome membrane [GO:0010008];
Golgi apparatus [GO:0005794];
Golgi membrane [GO:0000139];
lysosomal membrane [GO:0005765];
membrane [GO:0016020];
membrane coat [GO:0030117];
nucleoplasm [GO:0005654];
terminal bouton [GO:0043195]
Protein-protein interaction114455
Phylogenetic treeO14617
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.4521426878620060.1701260263632630.268975083326036
AZA vs. DISU-0.09362237969886310.7114167096175750.973233942294547
AZA vs. IL70.03520549684850650.8545246102995480.999311006273513
AZA vs. SAHA0.01327075954521320.9565837631348490.990779256780515
DISU vs. CD3-0.5581561207838630.1296250634783910.228534293201551
DISU vs. IL70.1195870941949050.6349110928988070.891519048621589
DISU vs. SAHA0.1089938297400920.7091542486199320.914054251030711
DMSO vs. AZA0.06850325826555190.6823362899906631
DMSO vs. CD3-0.3968231868780060.2192553978793010.32176398424675
DMSO vs. DISU0.1597879286362740.5122102071288830.912102132233928
DMSO vs. IL7-0.02565008795678530.8864166929209460.975829566927034
DMSO vs. SAHA-0.06071996244611130.7968942821350360.942933150490763
HIV vs. Mock in Activation0.2136605246184450.731396837550670.999983755607037
HIV vs. Mock in Latency-0.03505083952130750.8315098736649640.999834320637052
IL7 vs. CD3-0.4105149760302930.2050594494858720.328334794593726
SAHA vs. CD3-0.4629132635392150.1915956653832470.29191655821712
SAHA vs. IL7-0.0249553673376860.9183689835827130.969025203592735
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0522351 0.783051
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.964 0.947 0.997 1.062 0.994
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
4AFI X-ray 2.8Å A/B=680-729.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) enhanced by 22875976
Nef co-localizes with 10814565
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Envelope surface glycoprotein gp160; precursor co-localizes with 18076669
Nef binds 12486136
matrix co-localizes with 18076669
matrix interacts with 15766529
Pr55(Gag) co-localizes with 18076669
Pr55(Gag) complexes with 23125841
Pr55(Gag) interacts with 15766529
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)