Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003713
UniProt IDO00203
Primary gene name(s)AP3B1
Synonym gene name(s)ADTB3A
Protein nameAP-3 complex subunit beta-1
Protein functionSubunit of non-clathrin- and clathrin-associated adaptor protein complex 3, AP-3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network, TGN and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
Subcellular locationCytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250};
Peripheral membrane protein {ECO:0000250};
Cytoplasmic side {ECO:0000250}. Golgi apparatus {ECO:0000250}. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O00203
Gene Ontology
(Biological Process)
Complete annatation
anterograde axonal transport [GO:0008089];
anterograde synaptic vesicle transport [GO:0048490];
antigen processing and presentation, exogenous lipid antigen via MHC class Ib [GO:0048007];
blood coagulation [GO:0007596];
intracellular protein transport [GO:0006886];
melanosome organization [GO:0032438];
positive regulation of NK T cell differentiation [GO:0051138];
protein targeting to lysosome [GO:0006622]
Gene Ontology
(Molecular Function)
Complete annatation
GTP-dependent protein binding [GO:0030742];
protein phosphatase binding [GO:0019903]
Gene Ontology
(Cellular Component)
Complete annatation
AP-3 adaptor complex [GO:0030123];
axon cytoplasm [GO:1904115];
clathrin adaptor complex [GO:0030131];
clathrin-coated vesicle membrane [GO:0030665];
Golgi apparatus [GO:0005794];
lysosomal membrane [GO:0005765];
membrane [GO:0016020]
Protein-protein interaction114116
Phylogenetic treeO00203
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.5835637486195470.07695932680311550.141783519566869
AZA vs. DISU0.1613612590258920.524044793765930.94090051481386
AZA vs. IL70.2239251892209580.2460042249712550.995802196416289
AZA vs. SAHA-0.176719777743550.469976572423580.805139999171844
DISU vs. CD3-0.4345327333075580.2326055035008590.356729612348352
DISU vs. IL70.05344032501942180.8321293807921440.964820397354322
DISU vs. SAHA-0.3369701993596260.248561449960380.627062204946918
DMSO vs. AZA-0.01892576143218260.910506670571971
DMSO vs. CD3-0.6126677688291780.05710588677428960.107685590719073
DMSO vs. DISU-0.1819489651148920.4563700381115790.890973002147989
DMSO vs. IL70.2499221532397320.1662168378334790.674465684820684
DMSO vs. SAHA-0.1650572061829570.4850343052820510.806311632369356
HIV vs. Mock in Activation-0.1242732353404390.8420309025900490.999983755607037
HIV vs. Mock in Latency0.02103350763060560.899037853385860.999834320637052
IL7 vs. CD3-0.3514715283040710.2748831641034920.410391633570913
SAHA vs. CD3-0.7849032779474870.02830131281508820.0616687079414949
SAHA vs. IL7-0.4040541196783280.09880426351036720.28494442363008
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0670747 0.715092
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.023 0.886 0.981 1.018 0.968
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Pr55(Gag) enhanced by 22875976
Envelope surface glycoprotein gp160; precursor co-localizes with 18076669
matrix co-localizes with 18076669
Pr55(Gag) co-localizes with 18076669
Pr55(Gag) modulated by 19934039

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04142 Lysosome - Homo sapiens (human)