Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003652
UniProt IDQ9NP73
Primary gene name(s)ALG13
Synonym gene name(s)CXorf45, GLT28D1
Protein namePutative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13
Protein functionIsoform 1: Possible multifunctional enzyme with both glycosyltransferase and deubiquitinase activities.; FUNCTION: Isoform 2: May be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway.
Subcellular locationIsoform 2: Endoplasmic reticulum {ECO:0000305}. Note=Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NP73
Gene Ontology
(Biological Process)
Complete annatation
dolichol-linked oligosaccharide biosynthetic process [GO:0006488]
Gene Ontology
(Molecular Function)
Complete annatation
N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity [GO:0004577];
poly(A RNA binding [GO:0044822];
thiol-dependent ubiquitinyl hydrolase activity [GO:0036459]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum membrane [GO:0005789]
Protein-protein interaction122956
Phylogenetic treeQ9NP73
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.2818683948197820.3902085068936090.511633111431098
AZA vs. DISU-0.01412894968353430.9557165954761420.996649735504266
AZA vs. IL7-0.02911165902781150.8802530852080430.999311006273513
AZA vs. SAHA-0.679650086076550.01438088453285220.123942812586297
DISU vs. CD3-0.3087611580406350.3950568138670970.528589243052779
DISU vs. IL7-0.02363669864608280.9257309277184690.986022837026695
DISU vs. SAHA-0.6649069624541270.04445214617541180.261773801035185
DMSO vs. AZA-0.004330944034451580.9795222799503341
DMSO vs. CD3-0.2970024936256770.3539935187256960.468168936845106
DMSO vs. DISU0.008328928008776140.9729447257441130.995077522347534
DMSO vs. IL7-0.01766663717911480.9221426496905680.983935880239036
DMSO vs. SAHA-0.682627921497540.01244925025085390.103888510814296
HIV vs. Mock in Activation-0.2849122738305030.6472603589870440.999983755607037
HIV vs. Mock in Latency0.01192840578011640.9425376010774180.999834320637052
IL7 vs. CD3-0.3025455783698310.3473086988108690.484451602551936
SAHA vs. CD3-0.9866703538561390.005908872328211470.0164444517056596
SAHA vs. IL7-0.654826894009840.01587232492676360.0866881978065343
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.348043 0.0174492
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.002 1.059 1.116 1.304 1.156
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
219015_s_at 1.59 No downregulated in CD8+ cells

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Envelope surface glycoprotein gp120 processed by 2283726

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00510 N-Glycan biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)