Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003648
UniProt IDP04075
Primary gene name(s)ALDOA
Synonym gene name(s)ALDA
Protein nameFructose-bisphosphate aldolase A
Protein functionPlays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein, By similarity. {ECO:0000250}.
Subcellular locationCytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+. {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P04075
Gene Ontology
(Biological Process)
Complete annatation
actin filament organization [GO:0007015];
ATP biosynthetic process [GO:0006754];
canonical glycolysis [GO:0061621];
fructose 1,6-bisphosphate metabolic process [GO:0030388];
fructose metabolic process [GO:0006000];
gluconeogenesis [GO:0006094];
glycolytic process [GO:0006096];
muscle cell cellular homeostasis [GO:0046716];
platelet degranulation [GO:0002576];
protein homotetramerization [GO:0051289];
regulation of cell shape [GO:0008360];
striated muscle contraction [GO:0006941]
Gene Ontology
(Molecular Function)
Complete annatation
actin binding [GO:0003779];
cytoskeletal protein binding [GO:0008092];
fructose binding [GO:0070061];
fructose-bisphosphate aldolase activity [GO:0004332];
identical protein binding [GO:0042802];
poly(A RNA binding [GO:0044822];
tubulin binding [GO:0015631]
Gene Ontology
(Cellular Component)
Complete annatation
actin cytoskeleton [GO:0015629];
cytosol [GO:0005829];
extracellular exosome [GO:0070062];
extracellular region [GO:0005576];
extracellular space [GO:0005615];
I band [GO:0031674];
M band [GO:0031430];
membrane [GO:0016020];
nucleus [GO:0005634];
platelet alpha granule lumen [GO:0031093]
Protein-protein interaction106728
Phylogenetic treeP04075
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.8743419745194430.008249372101019730.0221907078898036
AZA vs. DISU0.2145822855325840.3956056242281170.902586488791209
AZA vs. IL70.138017781892340.4715937139134270.999311006273513
AZA vs. SAHA0.005548748128198530.9818045118404490.996243190432113
DISU vs. CD3-0.6716624280502510.06723221516805180.13692849683043
DISU vs. IL7-0.08615892703197620.7324413991381540.933273103880286
DISU vs. SAHA-0.2064005794669080.4785479756052640.810177099567168
DMSO vs. AZA-0.009021636896889030.9568895566970781
DMSO vs. CD3-0.8951407246087830.005491680752885090.0148531724492305
DMSO vs. DISU-0.2256483691971490.3553209106245260.845695250833134
DMSO vs. IL70.1545445112173870.3883946495105020.839072705842765
DMSO vs. SAHA0.009536993707008560.9676496349929960.993040349657116
HIV vs. Mock in Activation-0.02028205012610470.9739727013241060.999983755607037
HIV vs. Mock in Latency0.05600714508145130.7774182960822570.999834320637052
IL7 vs. CD3-0.7304357224888410.02367067238381290.0598896537949508
SAHA vs. CD3-0.8917529388507710.01243713357882870.0308421265481238
SAHA vs. IL7-0.134545363388390.5797740129770040.784094156793052
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.146968 0.856852
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.044 0.896 1.019 0.966 0.767
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB04326 1,3-Dihydroxyacetonephosphate experimental unknown unknown
DB02512 1,6-Fructose Diphosphate (Linear Form) experimental unknown unknown
DB04733 1,6-DI-O-PHOSPHONO-D-MANNITOL experimental unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1ALD X-ray 2.0Å A=2-364.
2ALD X-ray 2.1Å A=2-364.
4ALD X-ray 2.8Å A=2-364.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
retropepsin cleaves 22944692
Vpr downregulates 23874603

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00010 Glycolysis / Gluconeogenesis - Homo sapiens (human)
hsa00030 Pentose phosphate pathway - Homo sapiens (human)
hsa00051 Fructose and mannose metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01200 Carbon metabolism - Homo sapiens (human)
hsa01230 Biosynthesis of amino acids - Homo sapiens (human)
hsa04066 HIF-1 signaling pathway - Homo sapiens (human)