Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003613
UniProt IDQ9NRZ7
Primary gene name(s)AGPAT3
Synonym gene name(s)LPAAT3
Protein name1-acyl-sn-glycerol-3-phosphate acyltransferase gamma
Protein functionConverts lysophosphatidic acid, LPA into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor. Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA, PubMed:21173190. Has a preference for arachidonoyl-CoA as a donor. Has also a modest lysophosphatidylinositol acyltransferase, LPIAT activity, converts lysophosphatidylinositol, LPI into phosphatidylinositol, By similarity. {ECO:0000250|UniProtKB:Q9D517, ECO:0000269|PubMed:21173190}.
Subcellular locationEndoplasmic reticulum membrane {ECO:0000269|PubMed:20537980, ECO:0000269|PubMed:21173190};
Multi-pass membrane protein {ECO:0000269|PubMed:20537980}. Nucleus envelope.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9NRZ7
Gene Ontology
(Biological Process)
Complete annatation
CDP-diacylglycerol biosynthetic process [GO:0016024];
phosphatidic acid biosynthetic process [GO:0006654];
phospholipid biosynthetic process [GO:0008654];
triglyceride biosynthetic process [GO:0019432]
Gene Ontology
(Molecular Function)
Complete annatation
1-acylglycerol-3-phosphate O-acyltransferase activity [GO:0003841]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
Golgi membrane [GO:0000139];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
nuclear envelope [GO:0005635];
plasma membrane [GO:0005886]
Protein-protein interaction121224
Phylogenetic treeQ9NRZ7
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.8408155769040670.01085279706924390.027937379577736
AZA vs. DISU0.006288652224866590.9801604701487090.997994522186162
AZA vs. IL70.1865777847802010.3322240774221130.999311006273513
AZA vs. SAHA-0.089024381348220.7154342434288280.920271262209552
DISU vs. CD3-0.8468649221976120.02057192125355320.0526576907753543
DISU vs. IL70.1711381095795560.4967910011844630.824117650015759
DISU vs. SAHA-0.09322829467529630.7490060772617450.928754543934668
DMSO vs. AZA0.08339162271923850.6190616506205741
DMSO vs. CD3-0.7712042334665160.01668127039102060.0383903663979111
DMSO vs. DISU0.07467328489404120.7594727946793110.969857060964769
DMSO vs. IL70.1108388319124090.5377608236003090.896696737481162
DMSO vs. SAHA-0.1775044724256270.4518056197946530.782003654288608
HIV vs. Mock in Activation0.1630998208599950.7931549438816030.999983755607037
HIV vs. Mock in Latency-0.08207619023570880.6193065554598120.999834320637052
IL7 vs. CD3-0.6475693930407660.0453377910875720.101091201932392
SAHA vs. CD3-0.9536795896912220.007730558896008710.0206706037194555
SAHA vs. IL7-0.2785481412404430.2529741881460590.496398452501132
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0790543 0.642231
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.979 0.921 1.059 1.221 0.964
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00561 Glycerolipid metabolism - Homo sapiens (human)
hsa00564 Glycerophospholipid metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04072 Phospholipase D signaling pathway - Homo sapiens (human)