Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003604
UniProt IDQ99490
Primary gene name(s)AGAP2
Synonym gene name(s)CENTG1, KIAA0167
Protein nameArf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Protein functionGTPase-activating protein, GAP for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1, GRM1 to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1, AP-1-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. {ECO:0000269|PubMed:12640130, ECO:0000269|PubMed:14761976, ECO:0000269|PubMed:15118108, ECO:0000269|PubMed:16079295}.
Subcellular locationIsoform 1: Cytoplasm. Nucleus.;
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q99490
Gene Ontology
(Biological Process)
Complete annatation
negative regulation of neuron apoptotic process [GO:0043524];
negative regulation of protein catabolic process [GO:0042177];
protein transport [GO:0015031];
small GTPase mediated signal transduction [GO:0007264]
Gene Ontology
(Molecular Function)
Complete annatation
GTPase activator activity [GO:0005096];
GTP binding [GO:0005525];
metal ion binding [GO:0046872]
Gene Ontology
(Cellular Component)
Complete annatation
extracellular exosome [GO:0070062];
membrane [GO:0016020];
mitochondrion [GO:0005739];
nucleolus [GO:0005730];
nucleus [GO:0005634]
Protein-protein interaction125549
Phylogenetic treeQ99490
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.913050747717832.44137652316567e-082.64342147680697e-07
AZA vs. DISU-0.4504199838826420.07554348276744080.588957395980511
AZA vs. IL7-0.09259854940924430.6302039610884120.999311006273513
AZA vs. SAHA-0.1740734895341320.4756713176505870.807217611562026
DISU vs. CD31.451257339767720.0001161815674629580.000647438428072694
DISU vs. IL70.3480509228730370.1682345279315110.530832148779896
DISU vs. SAHA0.2787751264383740.3387846685178810.717888423324405
DMSO vs. AZA0.04374887290163310.7939867987715581
DMSO vs. CD31.947137069901735.85815895792763e-096.52760093943637e-08
DMSO vs. DISU0.4928298675948290.04400259516040330.418902600568597
DMSO vs. IL7-0.1292018625039920.4724233935301690.879015386011762
DMSO vs. SAHA-0.2234759202986660.343747027956380.695402310496196
HIV vs. Mock in Activation0.158131322953630.8233336599790810.999983755607037
HIV vs. Mock in Latency0.1146825636817870.487525788307950.999834320637052
IL7 vs. CD31.826073034967324.55835953427908e-086.03762330933115e-07
SAHA vs. CD31.716361452012483.521267204043e-062.50308947004377e-05
SAHA vs. IL7-0.08365950147899270.7317576732888780.878024241602841
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.33984 0.0202648
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.929 1.064 1.265 1.542 1.054
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
2BMJ X-ray 2.1Å A=402-577.
2IWR X-ray 1.5Å A=402-577.
2RLO NMR - A=674-914.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18187620

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04068 FoxO signaling pathway - Homo sapiens (human)
hsa04144 Endocytosis - Homo sapiens (human)