Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003594
UniProt IDP55263
Primary gene name(s)ADK
Synonym gene name(s)unknown
Protein nameAdenosine kinase
Protein functionATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Subcellular locationIsoform 1: Nucleus {ECO:0000269|PubMed:19635462}.;
SUBCELLULAR LOCATION: Isoform 2: Cytoplasm {ECO:0000269|PubMed:19635462}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: P55263
Gene Ontology
(Biological Process)
Complete annatation
adenosine salvage [GO:0006169];
AMP salvage [GO:0044209];
dATP biosynthetic process [GO:0006175];
purine-containing compound salvage [GO:0043101];
ribonucleoside monophosphate biosynthetic process [GO:0009156]
Gene Ontology
(Molecular Function)
Complete annatation
adenosine kinase activity [GO:0004001];
ATP binding [GO:0005524];
metal ion binding [GO:0046872];
poly(A RNA binding [GO:0044822]
Gene Ontology
(Cellular Component)
Complete annatation
cytoplasm [GO:0005737];
cytosol [GO:0005829];
nucleoplasm [GO:0005654]
Protein-protein interaction106644
Phylogenetic treeP55263
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      unknown
Brass et al., Science, 2008
      unknown
Smith et al., J. Immunol, 2010
      unknown
Interferon-stimulated
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model


DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD31.292186619948520.0001108713102176970.000529316562433048
AZA vs. DISU0.1926122032631680.4495554120365830.918736209468473
AZA vs. IL70.07384858909637890.7319428789579750.999311006273513
AZA vs. SAHA-0.1582018574558930.5207712895574090.833787192648792
DISU vs. CD3-1.112969864113620.002524474153808410.00915149315464508
DISU vs. IL7-0.1271383926388870.6161875849680910.883512896917278
DISU vs. SAHA-0.3503182046088570.2320772610359190.609334351976746
DMSO vs. AZA-0.05173369601905130.7678265404843171
DMSO vs. CD3-1.35906308631333.2541923157825e-050.00016517657424954
DMSO vs. DISU-0.2471967014572370.3144558086813150.81919072418999
DMSO vs. IL70.1330235579146460.4867778906159190.883479015294362
DMSO vs. SAHA-0.1133620114733340.6338609711319070.881590872640178
HIV vs. Mock in Activation-0.5275041184066260.3978854421865440.999983755607037
HIV vs. Mock in Latency-0.05521509502683180.7439365395515650.999834320637052
IL7 vs. CD3-1.20788963817160.000216201933611160.00114224035938346
SAHA vs. CD3-1.476390211682384.61157865320194e-050.000242639411926312
SAHA vs. IL7-0.2370400895609360.3350508755700910.585340206230807
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.437735 0.000305467
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.057 1.088 1.178 1.093 0.953
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
-1.23 0.009 -0.56 0.0233 0.2 0.3583 DNA recombination; repair and maintenance (8hpi)
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00640 Adenosine approved, investigational unknown substrate
DB00811 Ribavirin approved unknown substrate
DB00171 Adenosine triphosphate approved, nutraceutical unknown substrate
DB07173 7-(5-DEOXY-BETA-D-RIBOFURANOSYL)-5-IODO-7H-PYRROLO[2,3-D]PYRIMIDIN-4-AMINE experimental unknown unknown
DB07280 5-[4-(DIMETHYLAMINO)PHENYL]-6-[(6-MORPHOLIN-4-YLPYRIDIN-3-YL)ETHYNYL]PYRIMIDIN-4-AMINE experimental unknown unknown
DB00811 Ribavirin approved yes activator
DB00131 Adenosine monophosphate approved, nutraceutical unknown product of
DB01048 Abacavir approved, investigational unknown substrate

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1BX4 X-ray 1.5Å A=22-362.
2I6A X-ray 2.2Å A/B/C/D=22-362.
2I6B X-ray 2.3Å A/B=22-362.
4O1L X-ray 2.5Å A/B=17-362.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00230 Purine metabolism - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
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