Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003569
UniProt IDQ9ULC5
Primary gene name(s)ACSL5
Synonym gene name(s)ACS5, FACL5
Protein nameLong-chain-fatty-acid--CoA ligase 5
Protein functionAcyl-CoA synthetases, ACSL activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage, By similarity. Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids, By similarity. It was suggested that it may also stimulate fatty acid oxidation, By similarity. At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells. {ECO:0000250, ECO:0000269|PubMed:17681178, ECO:0000269|PubMed:18806831, ECO:0000269|PubMed:19459852}.
Subcellular locationMitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane {ECO:0000250};
Single-pass type III membrane protein {ECO:0000250}. Endoplasmic reticulum membrane {ECO:0000250};
Single-pass type III membrane protein {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9ULC5
Gene Ontology
(Biological Process)
Complete annatation
long-chain fatty acid metabolic process [GO:0001676];
long-chain fatty-acyl-CoA biosynthetic process [GO:0035338];
regulation of extrinsic apoptotic signaling pathway [GO:2001236]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
long-chain fatty acid-CoA ligase activity [GO:0004467]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
integral component of membrane [GO:0016021];
membrane [GO:0016020];
mitochondrial inner membrane [GO:0005743];
mitochondrial outer membrane [GO:0005741];
mitochondrion [GO:0005739];
nucleolus [GO:0005730];
nucleus [GO:0005634]
Protein-protein interaction119687
Phylogenetic treeQ9ULC5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7170013017579790.02960091034333080.0643602020621295
AZA vs. DISU-0.07700629161808130.7615539206717730.976021571236163
AZA vs. IL70.3587430102023380.06286550437442910.721684499189466
AZA vs. SAHA-1.14093533493440.002130453847947080.034755910502115
DISU vs. CD3-0.8055823981729280.02703674359281250.0657978483884494
DISU vs. IL70.4261773069542420.092585732152650.404645710598444
DISU vs. SAHA-1.06177129003730.01110402746501650.110461252755949
DMSO vs. AZA0.03989566337890330.8124594157210771
DMSO vs. CD3-0.6870086464740220.03279692841026080.0675441868080081
DMSO vs. DISU0.115427607947640.6373509504079290.946229564999022
DMSO vs. IL70.3258943921228290.07069198397809080.508569191842284
DMSO vs. SAHA-1.186838542833260.001393978637198370.0226630550606436
HIV vs. Mock in Activation-0.03183546396004350.9592271481530860.999983755607037
HIV vs. Mock in Latency0.1777592490606320.2829334362426740.999834320637052
IL7 vs. CD3-0.3517271894865510.2757300293608250.41133004083093
SAHA vs. CD3-1.880780009910714.97140975441734e-063.39769365075145e-05
SAHA vs. IL7-1.501501029844283.23530261899796e-050.000785329334601856
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.0802124 0.641688
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.021 1.255 1.348 1.547 1.074
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
capsid interacts with 24663101

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00061 Fatty acid biosynthesis - Homo sapiens (human)
hsa00071 Fatty acid degradation - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01212 Fatty acid metabolism - Homo sapiens (human)
hsa03320 PPAR signaling pathway - Homo sapiens (human)
hsa04146 Peroxisome - Homo sapiens (human)
hsa04920 Adipocytokine signaling pathway - Homo sapiens (human)