Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003567
UniProt IDO95573
Primary gene name(s)ACSL3
Synonym gene name(s)ACS3, FACL3, LACS3
Protein nameLong-chain-fatty-acid--CoA ligase 3
Protein functionAcyl-CoA synthetases, ACSL activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis, By similarity. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates, By similarity. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL, very low density lipoproteins. {ECO:0000250, ECO:0000269|PubMed:18003621}.
Subcellular locationMitochondrion outer membrane {ECO:0000250};
Single-pass type III membrane protein {ECO:0000250}. Peroxisome membrane {ECO:0000250};
Single-pass type III membrane protein {ECO:0000250}. Microsome membrane {ECO:0000250};
Single-pass type III membrane protein {ECO:0000250}. Endoplasmic reticulum membrane {ECO:0000250};
Single-pass type III membrane protein {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O95573
Gene Ontology
(Biological Process)
Complete annatation
brain development [GO:0007420];
fatty acid biosynthetic process [GO:0006633];
long-chain fatty acid import [GO:0044539];
long-chain fatty-acyl-CoA biosynthetic process [GO:0035338];
positive regulation of Golgi to plasma membrane protein transport [GO:0042998];
positive regulation of phosphatidylcholine biosynthetic process [GO:2001247];
positive regulation of secretion [GO:0051047];
response to nutrient [GO:0007584];
response to organic cyclic compound [GO:0014070];
very-low-density lipoprotein particle assembly [GO:0034379]
Gene Ontology
(Molecular Function)
Complete annatation
ATP binding [GO:0005524];
long-chain fatty acid-CoA ligase activity [GO:0004467];
protein domain specific binding [GO:0019904];
protein kinase binding [GO:0019901]
Gene Ontology
(Cellular Component)
Complete annatation
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
Golgi apparatus [GO:0005794];
integral component of membrane [GO:0016021];
lipid particle [GO:0005811];
membrane [GO:0016020];
mitochondrial outer membrane [GO:0005741];
perinuclear region of cytoplasm [GO:0048471];
peroxisomal membrane [GO:0005778]
Protein-protein interaction108477
Phylogenetic treeO95573
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD30.7319821929972610.02635305111314430.058436543530574
AZA vs. DISU0.003706836029467590.9883471995637070.998932793566471
AZA vs. IL70.1088690198971680.5736137316541220.999311006273513
AZA vs. SAHA-0.1999896246765260.4145100700969260.769498127665156
DISU vs. CD3-0.7409946583711210.0419372943600180.0937370663082609
DISU vs. IL70.09651712988921580.7024792395492990.923741138299926
DISU vs. SAHA-0.2031998854882330.4870239475972980.816731373385285
DMSO vs. AZA-0.1047853935751490.5356834888462711
DMSO vs. CD3-0.8488036371682570.008488077312528870.0216632524657266
DMSO vs. DISU-0.1104224387131560.6520869731663420.949713766615536
DMSO vs. IL70.2208113598402450.2227919961307170.730634444581427
DMSO vs. SAHA-0.1025063736716180.665078543111120.894993655464127
HIV vs. Mock in Activation-0.1437330770564650.8175874211832620.999983755607037
HIV vs. Mock in Latency-0.1425586114657360.3935521401711310.999834320637052
IL7 vs. CD3-0.6139920376940740.05713175479502840.122618103378252
SAHA vs. CD3-0.957323073516030.007342762292730010.0197775563452084
SAHA vs. IL7-0.3131332603008040.2005511148855930.433047916600119
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.0513237 0.793998
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.016 1.074 1.264 1.546 0.938
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00159 Icosapent approved, nutraceutical unknown inducer

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Vpu interacts with 22190034
Envelope surface glycoprotein gp120 complexes with 23125841
Gag-Pol complexes with 23125841
Pr55(Gag) complexes with 23125841
Nef complexes with 23125841

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00061 Fatty acid biosynthesis - Homo sapiens (human)
hsa00071 Fatty acid degradation - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa01212 Fatty acid metabolism - Homo sapiens (human)
hsa03320 PPAR signaling pathway - Homo sapiens (human)
hsa04146 Peroxisome - Homo sapiens (human)
hsa04920 Adipocytokine signaling pathway - Homo sapiens (human)