Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003559
UniProt IDO14734
Primary gene name(s)ACOT8
Synonym gene name(s)ACTEIII, PTE1, PTE2
Protein nameAcyl-coenzyme A thioesterase 8
Protein functionAcyl-coenzyme A, acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A, CoASH, providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH, PubMed:9299485, PubMed:9153233, PubMed:15194431. Competes with bile acid CoA:amino acid N-acyltransferase, BAAT for bile acid-CoA substrate, such as chenodeoxycholoyl-CoA. Shows a preference for medium-length fatty acyl-CoAs, C2 to C20, PubMed:9299485, PubMed:9153233. Inactive towards substrates with more than C20 aliphatic chains, PubMed:9153233. Involved in the metabolic regulation of peroxisome proliferation, PubMed:15194431. {ECO:0000269|PubMed:15194431, ECO:0000269|PubMed:9153233, ECO:0000269|PubMed:9299485}.; FUNCTION:, Microbial infection May mediate Nef-induced down-regulation of CD4 cell-surface expression, PubMed:9153233. {ECO:0000269|PubMed:9153233}.
Subcellular locationCytoplasm {ECO:0000269|PubMed:10092594}. Peroxisome matrix {ECO:0000269|PubMed:10092594, ECO:0000269|PubMed:15194431}. Note=Predominantly localized in the peroxisome, PubMed:10092594, PubMed:15194431. {ECO:0000269|PubMed:10092594, ECO:0000269|PubMed:15194431}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: O14734
Gene Ontology
(Biological Process)
Complete annatation
acyl-CoA metabolic process [GO:0006637];
alpha-linolenic acid metabolic process [GO:0036109];
bile acid biosynthetic process [GO:0006699];
dicarboxylic acid catabolic process [GO:0043649];
fatty acid beta-oxidation using acyl-CoA oxidase [GO:0033540];
long-chain fatty-acyl-CoA biosynthetic process [GO:0035338];
negative regulation of CD4 biosynthetic process [GO:0045225];
peroxisome fission [GO:0016559];
viral process [GO:0016032]
Gene Ontology
(Molecular Function)
Complete annatation
acetyl-CoA hydrolase activity [GO:0003986];
acyl-CoA hydrolase activity [GO:0047617];
carboxylic ester hydrolase activity [GO:0052689];
choloyl-CoA hydrolase activity [GO:0033882];
CoA hydrolase activity [GO:0016289];
medium-chain acyl-CoA hydrolase activity [GO:0052815];
palmitoyl-CoA hydrolase activity [GO:0016290];
receptor binding [GO:0005102]
Gene Ontology
(Cellular Component)
Complete annatation
mitochondrion [GO:0005739];
peroxisomal matrix [GO:0005782]
Protein-protein interaction115323
Phylogenetic treeO14734
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.8357643523239020.01286651008729660.0323422348291751
AZA vs. DISU-0.2161378972871530.3992000191395690.904305664226295
AZA vs. IL7-0.07567188931703660.7004543160682390.999311006273513
AZA vs. SAHA-0.163874532932930.5081453025646370.825812442825385
DISU vs. CD30.6071605009710570.102610541126790.190252312047385
DISU vs. IL70.1305544942566860.6089946012232140.880294717944189
DISU vs. SAHA0.05474044457785770.8524959606339620.960232245593492
DMSO vs. AZA0.06850120756775930.6913827606166411
DMSO vs. CD30.8907436720545280.006680064554535510.0175781960723279
DMSO vs. DISU0.2822705514492860.2534602166563970.771886382169071
DMSO vs. IL7-0.1365941198890650.457934742463730.872998531019969
DMSO vs. SAHA-0.2367861530786270.3221030293890010.675349854747028
HIV vs. Mock in Activation-0.1500056284302430.811517164972440.999983755607037
HIV vs. Mock in Latency0.07078191298303570.6750903120009760.999834320637052
IL7 vs. CD30.7659905931947020.0197298675270650.0518278656736581
SAHA vs. CD30.6491178202293760.07203996024519250.133930693424013
SAHA vs. IL7-0.09039179803267750.7143422194773980.869236498796512
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.359213 0.0263516
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
0.999 0.912 1.083 1.202 0.881
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
Nef interacts with 22190034
Nef binds 9153233

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00120 Primary bile acid biosynthesis - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)
hsa04146 Peroxisome - Homo sapiens (human)