Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003518
UniProt IDQ9UDR5
Primary gene name(s)AASS
Synonym gene name(s)unknown
Protein nameAlpha-aminoadipic semialdehyde synthase, mitochondrial
Protein functionBifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-ketoglutarate reductase and saccharopine dehydrogenase activity, respectively.
Subcellular locationMitochondrion {ECO:0000250}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9UDR5
Gene Ontology
(Biological Process)
Complete annatation
generation of precursor metabolites and energy [GO:0006091];
L-lysine catabolic process to acetyl-CoA via saccharopine [GO:0033512];
lysine biosynthetic process via aminoadipic acid [GO:0019878];
lysine catabolic process [GO:0006554];
protein tetramerization [GO:0051262]
Gene Ontology
(Molecular Function)
Complete annatation
saccharopine dehydrogenase, NAD+, L-glutamate-forming activity [GO:0047131];
saccharopine dehydrogenase, NADP+, L-lysine-forming activity [GO:0047130]
Gene Ontology
(Cellular Component)
Complete annatation
intracellular membrane-bounded organelle [GO:0043231];
mitochondrial matrix [GO:0005759];
mitochondrion [GO:0005739]
Protein-protein interaction115459
Phylogenetic treeQ9UDR5
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
      Yes - Two siRNA pools inhibit HIV replication and inhibition of Tat-mediated transactivation of the HIV LTR is not observed
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.5677855171364080.1240671428662450.209118762836059
AZA vs. DISU-0.06635997417503040.8241973039498380.98478550430575
AZA vs. IL70.03557264154158220.8874486514892130.999311006273513
AZA vs. SAHA0.8291508698343840.003498170107788410.0488706359636724
DISU vs. CD30.4895217249513260.2183758890055690.340695316794655
DISU vs. IL70.09438834343726220.7484540526509450.939198389465636
DISU vs. SAHA0.8955210705621940.005497066887729770.06950192539008
DMSO vs. AZA0.2514217203685940.2756268270360021
DMSO vs. CD30.8065150719822470.02370373873742350.0515961312063697
DMSO vs. DISU0.315482048724720.266610482888940.785013262693934
DMSO vs. IL7-0.2063945903044050.3744183035680850.831519022414553
DMSO vs. SAHA0.5703147100565420.03411599795460850.200645799817287
HIV vs. Mock in Activation0.3352037649605360.6264761996037270.999983755607037
HIV vs. Mock in Latency0.0567538821573270.8537289229122770.999834320637052
IL7 vs. CD30.6119556208423910.0903212838249010.175011275368651
SAHA vs. CD31.371782426362360.0003995917706886010.00161184367887506
SAHA vs. IL70.7857270777411260.005042340993270940.0386417123195558
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) unknown
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK 0.328618 0.0866743
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.051 1.067 1.321 1.472 1.156
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

Drugbank ID Drug Name Drug Status Pharmacological Action Drug Action
DB00157 NADH nutraceutical unknown unknown
DB02338 Nadph Dihydro-Nicotinamide-Adenine-Dinucleotidephosphate experimental unknown unknown
DB04207 N-(5-Amino-5-Carboxypentyl)Glutamic Acid experimental unknown unknown
DB00142 L-Glutamic Acid nutraceutical unknown unknown

Protein Secondary Structure       (annotations from PDB)      top

not found

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

HIV Partner Interaction Type PubMed
HIV-1 virus replication enhanced by expression of human gene 18976975

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa00310 Lysine degradation - Homo sapiens (human)
hsa01100 Metabolic pathways - Homo sapiens (human)