Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003392
UniProt IDQ5T4F4
Primary gene name(s)ZFYVE27
Synonym gene name(s)SPG33
Protein nameProtrudin
Protein functionKey regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport, PubMed:17082457. Promotes axonal elongation and contributes to the establishment of neuronal cell polarity, By similarity. Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner, PubMed:19289470. Contributes to both the formation and stabilization of the tubular ER network, PubMed:24668814. Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections, PubMed:23969831. Acts as an adapter protein and facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner, PubMed:21976701. {ECO:0000250|UniProtKB:Q3TXX3, ECO:0000269|PubMed:17082457, ECO:0000269|PubMed:19289470, ECO:0000269|PubMed:21976701, ECO:0000269|PubMed:23969831, ECO:0000269|PubMed:24668814}.
Subcellular locationRecycling endosome membrane {ECO:0000250|UniProtKB:Q6P7B7};
Multi-pass membrane protein {ECO:0000255}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:19289470, ECO:0000269|PubMed:23969831, ECO:0000269|PubMed:24668814};
Multi-pass membrane protein {ECO:0000269|PubMed:23969831}. Cell projection, growth cone membrane {ECO:0000250|UniProtKB:Q3TXX3};
Multi-pass membrane protein {ECO:0000255}. Note=Localizes at both dendrites and axons, By similarity. Localizes to endoplasmic reticulum tubular network. {ECO:0000250|UniProtKB:Q3TXX3, ECO:0000269|PubMed:23969831, ECO:0000269|PubMed:24668814}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q5T4F4
Gene Ontology
(Biological Process)
Complete annatation
endoplasmic reticulum tubular network assembly [GO:0071787];
neuron projection development [GO:0031175];
neurotrophin TRK receptor signaling pathway [GO:0048011];
positive regulation of axon extension [GO:0045773];
protein localization to plasma membrane [GO:0072659];
vesicle-mediated transport [GO:0016192]
Gene Ontology
(Molecular Function)
Complete annatation
metal ion binding [GO:0046872]
Gene Ontology
(Cellular Component)
Complete annatation
axon [GO:0030424];
dendrite [GO:0030425];
endoplasmic reticulum [GO:0005783];
endoplasmic reticulum membrane [GO:0005789];
endoplasmic reticulum tubular network [GO:0071782];
growth cone membrane [GO:0032584];
integral component of membrane [GO:0016021];
recycling endosome membrane [GO:0055038]
Protein-protein interaction125623
Phylogenetic treeQ5T4F4
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-0.2444460081033390.4561792155298010.57711501455483
AZA vs. DISU0.1790323703732830.4801653597359740.931293442184775
AZA vs. IL7-0.2430178733503940.2091350384268160.9741323962322
AZA vs. SAHA-0.1118859199989910.6475078224647160.892039351125213
DISU vs. CD30.4106643642588560.2589217581602780.386485476423269
DISU vs. IL7-0.4316368698597440.08803068284075160.392055100429897
DISU vs. SAHA-0.2883741684110190.3235063939005520.706398849967432
DMSO vs. AZA0.04578796854917350.786072366951251
DMSO vs. CD30.2775071240354560.3863783567127090.500825761634448
DMSO vs. DISU-0.1353626254501530.579848645579530.932467317571925
DMSO vs. IL7-0.281291122067440.1201884184580080.611275767341979
DMSO vs. SAHA-0.1625106660530020.4918802845430520.809205083325874
HIV vs. Mock in Activation0.02574263109581340.9670267254355640.999983755607037
HIV vs. Mock in Latency0.1193273155523920.4720905179845870.999834320637052
IL7 vs. CD30.00695807142364260.9827425297715510.989438123789098
SAHA vs. CD30.109229527621330.7573823545972610.826125657482681
SAHA vs. IL70.1289336938555990.5976849418817840.79535342122114
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
0.5649 0.0264

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.101495 0.55208
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
unknown unknown unknown unknown unknown
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1X4U NMR - A=341-411.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)