Detailed entry information

Protein Information (annotations from UniProt)

Database IDHIV0003391
UniProt IDQ9H1K0
Primary gene name(s)RBSN
Synonym gene name(s)ZFYVE20
Protein nameRabenosyn-5
Protein functionRab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of CTSD/cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, PtdInsP3, PubMed:11062261, PubMed:11788822, PubMed:15020713. Plays a role in the recycling of transferrin receptor to the plasma membrane, PubMed:22308388. {ECO:0000269|PubMed:11062261, ECO:0000269|PubMed:11788822, ECO:0000269|PubMed:15020713, ECO:0000269|PubMed:22308388}.
Subcellular locationCell membrane;
Cytoplasmic side. Early endosome membrane {ECO:0000269|PubMed:22308388};
Lipid-anchor. Note=Enriched in endosomes that are in close proximity to clathrin-enriched regions at the cell surface. {ECO:0000269|PubMed:22308388}.
ECO codeClick here for more information.
Amino acid sequence
FASTA format: Q9H1K0
Gene Ontology
(Biological Process)
Complete annatation
blood coagulation [GO:0007596];
early endosome to Golgi transport [GO:0034498];
endocytosis [GO:0006897];
endosomal transport [GO:0016197];
endosomal vesicle fusion [GO:0034058];
Golgi to endosome transport [GO:0006895];
Golgi to lysosome transport [GO:0090160];
Golgi to vacuole transport [GO:0006896];
protein transport [GO:0015031];
regulation of Golgi organization [GO:1903358];
vacuole inheritance [GO:0000011]
Gene Ontology
(Molecular Function)
Complete annatation
Gene Ontology
(Cellular Component)
Complete annatation
Protein-protein interaction122084
Phylogenetic treeQ9H1K0
HIV replication factor status Zhou et al., Cell. Host. Microbe., 2008
Brass et al., Science, 2008
Smith et al., J. Immunol, 2010
gene status
Lu et al., J. Virol., 2011
      Folds changes 8h: unknown; Folds changes 16h: unknown; Tested: unknown;
Schoggins JW and Rice CM, Curr. Opin. Virol., 2011
      Targeted viruses: unknown
      Viral life cycle: unknown
      Mechanism related to antiviral activity: unknown
Anti-viral restriction factor Liu et al., Retrovirology, 2011
      unknown (Triplicates)

Gene Expression Profile       top

            Up-regulated;            Down-regulated

For brief introduction to each study, please go to the help page.

Gene expression during HIV latency

(1). Mohammadi et al., PLoS Pathog., 2014

Differentially expressed transcripts (Pairwise) during latency and subsequent viral reactivation using several agents - Primary CD4+ T-cell based model

DMSO: Dimethyl suloxyde (negative control) - 0.0033% final
SAHA: Vorinostat (Histone deacetylase inhibitor) - 0.5 μM
CD3: TCR Stimulation by IL-2+ antiCD3/anti-CD28 antibodies
IL7: Interleukin-7 based stimulation
DISU: Disulfiram (alcohol dehydrogenase inhibitor) - 0.5 μM
AZA: 5-azacytidine (AZA; DNA methylation inhibitor) - 1 μM
Experimental Condition Log2 Fold Change P value Adjusted P value
AZA vs. CD3-1.218939875546240.0002547220750257480.00109753319462209
AZA vs. DISU0.1445721675605450.5687671308263420.954304256505839
AZA vs. IL7-0.09461547938370240.6250827406222250.999311006273513
AZA vs. SAHA-0.1721126432978530.4825171505082430.811065327688238
DISU vs. CD31.350086158166560.000256790879769930.00128764994545412
DISU vs. IL7-0.2480342524312440.3262852427560620.707117172787051
DISU vs. SAHA-0.3152182979844530.2806297455275270.66181242320829
DMSO vs. AZA-0.009774179910448960.9539024640169421
DMSO vs. CD31.195050517915780.0002375137944157220.000970476081603704
DMSO vs. DISU-0.1568727790426740.5214055370687890.914921957877669
DMSO vs. IL7-0.07721145064326890.6698870530607460.93213494903488
DMSO vs. SAHA-0.1682872254890040.4772044604459660.801599244692505
HIV vs. Mock in Activation0.1255374484346790.8407810532506180.999983755607037
HIV vs. Mock in Latency-0.01137835158001680.9453952821011980.999834320637052
IL7 vs. CD31.131989018616040.0005192222119677630.002419238454711
SAHA vs. CD31.021654844952680.004294657879486240.0125354433941231
SAHA vs. IL7-0.08168468093393590.7383024013760830.881056555732713
(2). Iglesias-Ussel et al., J. Virol., 2013

Up and Downregulated transcripts during Latency (Latently infected CD4+ T cells vs Uninfected)- Primary CD4+Tcell based model
Log2 Fold Change P Value
unknown unknown

Gene expression during HIV infection and replication

(1). Imbeault et al., PloS Pathog., 2012

Transcriptomic profiling of HIV-1 infected CD4+ T cells - Primary CD4+ T cells
Experiment type Log2 Fold Change P Value Adjusted P Value
Infected vs. Mock unknown unknown unknown
Infected vs. Bystander unknown unknown unknown
(2). Lefebvre et al., J. Virol., 2011

Transcriptome analysis of T-cell line (Sup T1)
Log2 Fold Change unknown
(3). Li et al., J. Immunol., 2013

Lymphatic tissue
Acute Fold Change Acute P Value Asymt Fold Change Asypt P Value AIDS Fold Change AIDS P Value
unknown unknown unknown unknown unknown unknown
(4). Chang et al., MBio., 2011

Transcriptome analysis of T-cell line (Sup T1)

Derived from Sherrill-Mix et al., BMC Retrovirol., 2015 cross validation
Up-regulated (True) TRUE
(5). Sherrill-Mix et al., BMC Retrovirol., 2015

Deep RNA-seq analysis of primary human T cell infected with low passage HIV isolate HIV89.6 - Primary CD4+ T cell based
Test Status Log2 Fold Change P Value
OK -0.172654 0.259392
(6). Rotger et al., PLoS Pathog., 2010

Genome-wide mRNA expression of CD4+ T cells from HIV-infected patient
(Genes differentially expressed (at adjusted p<0.01) according to the empirical Bayes approach)
Log2 Fold Change P Value
unknown unknown

Proteomic/Transcriptomics studies indicating differentially expressed genes mediated by HIV

(1). Greenwood et al., Elife, 2016

Activated (CD3/CD28) Primary human CD4+ T cells infected with pNL4-3-dE-EGFP. The table shows the complete (unfiltered) TMT (tandem mass tag)-based proteomic time course dataset
6 h 24 h 48 h 72 h RTi
1.033 0.726 1 1.05 0.916
(2). Navare et al., Virology, 2012

SUP-T1 cell line
FC-4hpi P-value FC-8hpi P-value FC-20hpi P-value Category
unknown unknown unknown unknown unknown unknown unknown
(3). Hyrcza et al., J. Virolo., 2007

Primary human CD4+ and CD8+ T Cells
Affymetrix Prob ID Fold Change In CD8? Category
unknown unknown unknown unknown

Protein Overview       top

Drug-protein Interaction       (annotations from DrugBank)      top

not found

Protein Secondary Structure       (annotations from PDB)      top

PDB Accession Method Resolution Chain Structure Preview
1YZM X-ray 1.5Å A=458-503.
1Z0J X-ray 1.3Å B=728-784.
1Z0K X-ray 1.9Å B/D=440-503.

HIV-1 Interaction       (annotations from NCBI HIV-1 Interaction Database)      top

not found

Metabolic/Signalling Pathway       (annotations from KEGG database)      top

Pathway Accession Number Description
hsa04144 Endocytosis - Homo sapiens (human)